Precision editing of large animal genomes
Transgenic animals are an important source of protein and nutrition for most humans and
will play key roles in satisfying the increasing demand for food in an ever-increasing world …
will play key roles in satisfying the increasing demand for food in an ever-increasing world …
Completion of the swine genome will simplify the production of swine as a large animal biomedical model
Background Anatomic and physiological similarities to the human make swine an excellent
large animal model for human health and disease. Methods Cloning from a modified …
large animal model for human health and disease. Methods Cloning from a modified …
Hyperactive piggyBac Gene Transfer in Human Cells and In Vivo
We characterized a recently developed hyperactive piggyBac (pB) transposase enzyme
[containing seven mutations (7pB)] for gene transfer in human cells in vitro and to somatic …
[containing seven mutations (7pB)] for gene transfer in human cells in vitro and to somatic …
Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis
J Marh, Z Stoytcheva, J Urschitz… - Proceedings of the …, 2012 - National Acad Sciences
We have developed a unique method for mouse transgenesis. The transposase-enhanced
pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac …
pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac …
The Sleeping Beauty Transposon Toolbox
The mobility of class II transposable elements (DNA transposons) can be experimentally
controlled by separating the two functional components of the transposon: the terminal …
controlled by separating the two functional components of the transposon: the terminal …
Development of transgenic cloned pig models of skin inflammation by DNA transposon-directed ectopic expression of human β1 and α2 integrin
NH Staunstrup, J Madsen, MN Primo, J Li, Y Liu… - PLoS …, 2012 - journals.plos.org
Integrins constitute a superfamily of transmembrane signaling receptors that play pivotal
roles in cutaneous homeostasis by modulating cell growth and differentiation as well as …
roles in cutaneous homeostasis by modulating cell growth and differentiation as well as …
Insertional mutagenesis by a hybrid piggyBac and sleeping beauty transposon in the rat
K Furushima, CW Jang, DW Chen, N Xiao… - Genetics, 2012 - academic.oup.com
Abstract A hybrid piggyBac/Sleeping Beauty transposon-based insertional mutagenesis
system that can be mobilized by simple breeding was established in the rat. These …
system that can be mobilized by simple breeding was established in the rat. These …
Transposable elements as tools for reshaping the genome: it is a huge world after all!
S Bire, F Rouleux-Bonnin - Mobile Genetic Elements: Protocols and …, 2012 - Springer
Transposable elements (TEs) are discrete pieces of DNA that can move from one site to
another within genomes and sometime between genomes. They are found in all major …
another within genomes and sometime between genomes. They are found in all major …
Production of biopharmaceuticals in transgenic animals
H Niemann, A Kind, A Schnieke - … : drug discovery and clinical …, 2012 - Wiley Online Library
Farm animals and their products have long made valuable contributions to human nutrition,
clothing, labor, research, development, and medicine, greatly improving human health and …
clothing, labor, research, development, and medicine, greatly improving human health and …
in vivo models for defining molecular subtypes of the primitive neuroectodermal tumor genome: current challenges and solutions
JD Larson, DA Largaespada - in vivo, 2012 - iv.iiarjournals.org
Primitive neuroectodermal tumors (PNET) of the brain include medulloblastoma (MB) and
central nervous system primitive neuroectodermal tumor (CNS PNET) subtypes, which share …
central nervous system primitive neuroectodermal tumor (CNS PNET) subtypes, which share …