Nicotiana benthamiana is the most widely-used experimental host in plant virology. The
recent release of the draft genome sequence for N. benthamiana consolidates its role as a …

Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels.
Accurate and reproducible results are dependent on the correct choice of the reference …

实时荧光定量PCR (qRT-PCR) 具有灵敏度高, 特异性强, 重复的动态定量范围和高通量等优点,
是进行植物基因表达和转录分析最常用的技术手段之一. 选择合适的内参基因是正确运用实时 …

Gene expression analysis by reverse transcriptase real-time or quantitative polymerase
chain reaction (RT-qPCR) is becoming widely used for non-model plant species. Given the …

Introduction Cotton is an important economic crop. Genetic, developmental and molecular
studies of cotton require high‐quality total RNA from different tissues. Due to the richness in …

Jasmonic acid (JA) is a well-characterized phytohormone that acts in various ways to
influence plant development. Its role in cotton fiber development, however, has not yet been …

The MADS-box gene plays a crucial role in the formation of the floral meristem, development
of floral organs and florescence regulation in plants. Based on a full-length cDNA library of …