Miniaturized nucleic acid amplification systems for rapid and point-of-care diagnostics: a review
F Ahmad, SA Hashsham - Analytica chimica acta, 2012 - Elsevier
Point-of-care (POC) genetic diagnostics critically depends on miniaturization and integration
of sample processing, nucleic acid amplification, and detection systems. Polymerase chain …
of sample processing, nucleic acid amplification, and detection systems. Polymerase chain …
Gene-Z: a device for point of care genetic testing using a smartphone
By 2012, point of care (POC) testing will constitute roughly one third of the $59 billion in vitro
diagnostics market. The ability to carry out multiplexed genetic testing and wireless …
diagnostics market. The ability to carry out multiplexed genetic testing and wireless …
Development of TaqMan probe-based insulated isothermal PCR (iiPCR) for sensitive and specific on-site pathogen detection
YL Tsai, HTT Wang, HFG Chang, CF Tsai, CK Lin… - 2012 - journals.plos.org
Insulated isothermal PCR (iiPCR), established on the basis of Ralyeigh-Bénard convection,
is a rapid and low-cost platform for nucleic acid amplification. However, the method used for …
is a rapid and low-cost platform for nucleic acid amplification. However, the method used for …
Quantitative and sensitive protein detection strategies based on aptamers
Y Yoshida, I Waga, K Horii - PROTEOMICS–Clinical …, 2012 - Wiley Online Library
Aptamers are functional oligonucleotides of single‐stranded RNA or DNA that can
selectively recognize their targets with high affinity. Hence, they have been widely …
selectively recognize their targets with high affinity. Hence, they have been widely …
Solid-phase pcr in a picowell array for immobilizing and arraying 100000 pcr products to a microscope slide
We present a method for performing highly parallel PCR reactions in a picowell array (PWA)
simultaneously immobilizing generated PCR products in a covalent and spatially-resolved …
simultaneously immobilizing generated PCR products in a covalent and spatially-resolved …
Fast nucleic acid amplification for integration in point‐of‐care applications
S Brunklaus, TE Hansen‐Hagge, J Erwes… - …, 2012 - Wiley Online Library
An ultrafast microfluidic PCR module (30 PCR cycles in 6 min) based on the oscillating fluid
plug concept was developed. A robust amplification of native genomic DNA from whole …
plug concept was developed. A robust amplification of native genomic DNA from whole …
Plug-and-play, infrared, laser-mediated PCR in a microfluidic chip
Microfluidic polymerase chain reaction (PCR) systems have set milestones for small volume
(100 nL–5 μL), amplification speed (100–400 s), and on-chip integration of upstream and …
(100 nL–5 μL), amplification speed (100–400 s), and on-chip integration of upstream and …
Flow-through PCR on a 3D qiandu-shaped polydimethylsiloxane (PDMS) microdevice employing a single heater: toward microscale multiplex PCR
W Wu, NY Lee - Analyst, 2012 - pubs.rsc.org
Consistent temperature control in an on-chip flow-through polymerase chain reaction (PCR)
employing two or more heaters is one of the main obstacles for device miniaturization and …
employing two or more heaters is one of the main obstacles for device miniaturization and …
Competitive reporter monitored amplification (CMA)-quantification of molecular targets by real time monitoring of competitive reporter hybridization
T Ullrich, E Ermantraut, T Schulz, K Steinmetzer - PloS one, 2012 - journals.plos.org
Background State of the art molecular diagnostic tests are based on the sensitive detection
and quantification of nucleic acids. However, currently established diagnostic tests are …
and quantification of nucleic acids. However, currently established diagnostic tests are …
Real-time droplet DNA amplification with a new tablet platform
SL Angione, A Chauhan, A Tripathi - Analytical chemistry, 2012 - ACS Publications
We present a novel droplet-based tablet platform for temporal polymerase chain reaction
(PCR) in microliter droplets. The simple design of the device does not require extensive …
(PCR) in microliter droplets. The simple design of the device does not require extensive …