Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment

N Ichihashi, K Usui, Y Kazuta, T Sunami… - Nature …, 2013 - nature.com
The ability to evolve is a key characteristic that distinguishes living things from non-living
chemical compounds. The construction of an evolvable cell-like system entirely from non …

Macromolecular interactions of the bacterial division FtsZ protein: from quantitative biochemistry and crowding to reconstructing minimal divisomes in the test tube

G Rivas, C Alfonso, M Jiménez, B Monterroso… - Biophysical …, 2013 - Springer
The division of Escherichia coli is an essential process strictly regulated in time and space. It
requires the association of FtsZ with other proteins to assemble a dynamic ring during …

Evolving protocells to prototissues: rational design of a missing link

S Mantri, KT Sapra - Biochemical Society Transactions, 2013 - portlandpress.com
Realization of a functional artificial cell, the so-called protocell, is a major challenge posed
by synthetic biology. A subsequent goal is to use the protocellular units for the bottom-up …

Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free Synthetic Biology

R Lentini, M Forlin, L Martini, C Del Bianco… - ACS synthetic …, 2013 - ACS Publications
To facilitate the construction of cell-free genetic devices, we evaluated the ability of 17
different fluorescent proteins to give easily detectable fluorescence signals in real-time from …

The encapsulation of cell-free transcription and translation machinery in vesicles for the construction of cellular mimics

AC Spencer, P Torre, SS Mansy - JoVE (Journal of Visualized …, 2013 - jove.com
As interest shifts from individual molecules to systems of molecules, an increasing number of
laboratories have sought to build from the bottom up cellular mimics that better represent the …