[HTML][HTML] Environmental assessment of enzyme use in industrial production–a literature review

KR Jegannathan, PH Nielsen - Journal of cleaner production, 2013 - Elsevier
Enzymatic processes have been implemented in a broad range of industries in recent
decades because they are specific, fast in action and often save raw materials, energy …

Biotechnology of non-Saccharomyces yeasts—the ascomycetes

EA Johnson - Applied microbiology and biotechnology, 2013 - Springer
Saccharomyces cerevisiae and several other yeast species are among the most important
groups of biotechnological organisms. S. cerevisiae and closely related ascomycetous …

Strategies for stabilization of enzymes in organic solvents

V Stepankova, S Bidmanova, T Koudelakova… - Acs …, 2013 - ACS Publications
One of the major barriers to the use of enzymes in industrial biotechnology is their
insufficient stability under processing conditions. The use of organic solvent systems instead …

Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel

T Koudelakova, R Chaloupkova… - Angewandte …, 2013 - Wiley Online Library
Biocatalysts produced by mesophilic organisms exhibit high selectivity and operability under
environmentally friendly conditions.[1] However, their practical applications can be limited by …

Self-assembly of synthetic metabolons through synthetic protein scaffolds: one-step purification, co-immobilization, and substrate channeling

C You, YHP Zhang - ACS synthetic biology, 2013 - ACS Publications
One-step purification of a multi-enzyme complex was developed based on a mixture of cell
extracts containing three dockerin-containing enzymes and one family 3 cellulose-binding …

Directed evolution as a powerful synthetic biology tool

RE Cobb, N Sun, H Zhao - Methods, 2013 - Elsevier
At the heart of synthetic biology lies the goal of rationally engineering a complete biological
system to achieve a specific objective, such as bioremediation and synthesis of a valuable …

Biocatalytic Asymmetric Alkene Reduction: Crystal Structure and Characterization of a Double Bond Reductase from Nicotiana tabacum

DJ Mansell, HS Toogood, J Waller, JMX Hughes… - ACS …, 2013 - ACS Publications
The application of biocatalysis for the asymmetric reduction of activated C C is a powerful
tool for the manufacture of high-value chemical commodities. The biocatalytic potential of “ …

[HTML][HTML] A highly productive, whole-cell DERA chemoenzymatic process for production of key lactonized side-chain intermediates in statin synthesis

M Ošlaj, J Cluzeau, D Orkić, G Kopitar, P Mrak… - PLoS …, 2013 - journals.plos.org
Employing DERA (2-deoxyribose-5-phosphate aldolase), we developed the first whole-cell
biotransformation process for production of chiral lactol intermediates useful for synthesis of …

Cell-free biosystems for biomanufacturing

C You, YHP Zhang - Future trends in biotechnology, 2013 - Springer
Although cell-free biosystems have been used as a tool for investigating fundamental
aspects of biological systems for more than 100 years, they are becoming an emerging …

[HTML][HTML] Concentration by ultrafiltration and stabilization of phytase produced by solid-state fermentation

DE Rodríguez-Fernández, JL Parada… - Process …, 2013 - Elsevier
Ultrafiltration is an attractive downstream processing technique for concentrating enzymes
and could be considered the primary step of purification. However, the efficiency of this …