[HTML][HTML] Photoactivatable lipid probes for studying biomembranes by photoaffinity labeling

Y Xia, L Peng - Chemical reviews, 2013 - ACS Publications
1.1. Biomembranes Biological membranes consist mainly of lipids and proteins (Figure 1)
and form the boundaries of all cells. The major lipids present in biomembranes are …

[HTML][HTML] Comparative and quantitative global proteomics approaches: an overview

B Deracinois, C Flahaut, S Duban-Deweer… - Proteomes, 2013 - mdpi.com
Proteomics became a key tool for the study of biological systems. The comparison between
two different physiological states allows unravelling the cellular and molecular mechanisms …

Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display

BJ Tillotson, YK Cho, EV Shusta - Methods, 2013 - Elsevier
Membrane proteins (MPs) are often desirable targets for antibody engineering. However, the
majority of antibody engineering platforms depend implicitly on aqueous solubility of the …

Correction factors for membrane protein molecular weight readouts on sodium dodecyl sulfate–polyacrylamide gel electrophoresis

A Rath, CM Deber - Analytical biochemistry, 2013 - Elsevier
Membrane proteins are known to migrate anomalously on sodium dodecyl sulfate–
polyacrylamide gel electrophoresis (SDS–PAGE) to relative molecular mass (Mr) values …

Proteomic tools for environmental microbiology—a roadmap from sample preparation to protein identification and quantification

L Wöhlbrand, K Trautwein, R Rabus - Proteomics, 2013 - Wiley Online Library
The steadily increasing amount of (meta‐) genomic sequence information of diverse
organisms and habitats has a strong impact on research in microbial physiology and …

Proteomic analysis of Mesembryanthemum crystallinum leaf microsomal fractions finds an imbalance in V-ATPase stoichiometry during the salt-induced transition …

C Cosentino, D Di Silvestre… - Biochemical …, 2013 - portlandpress.com
The halophyte Mesembryanthemum crystallinum adapts to salt stress by salt uptake and
switching from C3 photosynthesis to CAM (crassulacean acid metabolism). An important role …

Myelin proteome analysis: methods and implications for the myelin cytoskeleton

O Jahn, S Tenzer, N Bartsch, J Patzig… - The Cytoskeleton: Imaging …, 2013 - Springer
Myelin, the multilayered membrane that enwraps and insulates neuronal axons for fast
signal propagation, is a plasma membrane specialization of oligodendrocytes and Schwann …

Identification of autoantigens in body fluids by combining pull-downs and organic precipitations of intact immune complexes with quantitative label-free mass …

J Merl, CA Deeg, ME Swadzba, M Ueffing… - Journal of proteome …, 2013 - ACS Publications
Most autoimmune diseases are multifactorial diseases and are caused by the
immunological reaction against a number of autoantigens. Key for understanding …

Multidimensional protein identification technology for direct-tissue proteomics of heart

D Di Silvestre, F Brambilla, PL Mauri - Heart Proteomics: Methods and …, 2013 - Springer
Multidimensional protein identification technology (MudPIT) is an invaluable approach to
identify proteins at large-scale level. Here, we describe a procedure of investigation to …

水稻剑叶类囊体膜蛋白复合物的蓝绿温和胶电泳分析

叶露幻, 郑宝刚, 沈唯军, 陈国祥, 吕川根 - 江苏农业科学, 2013 - cqvip.com
以水稻两优培九剑叶为材料, 通过蓝绿温和胶电泳对其类囊体膜蛋白复合物进行分析.
亚细胞组分是植物蛋白质组学研究的重点, 而高度疏水且结构复杂的膜蛋白复合物在传统二维 …