Decline in baculovirus-expressed recombinant protein production with increasing cell density is strongly correlated to impairment of virus replication and mRNA …

HT Huynh, TTB Tran, LCL Chan, LK Nielsen… - Applied microbiology and …, 2013 - Springer
The cell density effect is a well-established constraint in the baculovirus–insect cell
expression platform, in which cell-specific productivity declines with increasing cell density …

The effect of spent medium recycle on cell proliferation, metabolism and baculovirus production by the lepidopteran Se301 cell line infected at very low MOI

A Beas-Catena, A Sánchez-Mirón… - … of microbiology and …, 2013 - koreascience.kr
The aim of this paper was to study the effect of spent medium recycle on Spodoptera exigua
Se301 cell line proliferation, metabolism, and baculovirus production when grown in batch …

Increase of protein yield in High Five cells in a single‐use perfusion bioreactor by medium replacement

M Rausch, R Pörtner, J Knäblein - Chemie Ingenieur Technik, 2013 - Wiley Online Library
A multitude of heterologous proteins is needed in pharmaceutical research. These have to
be produced in robust and flexible cultivation systems to allow a fast and cost‐effective …