The new frontier of genome engineering with CRISPR-Cas9
JA Doudna, E Charpentier - Science, 2014 - science.org
Background Technologies for making and manipulating DNA have enabled advances in
biology ever since the discovery of the DNA double helix. But introducing site-specific …
biology ever since the discovery of the DNA double helix. But introducing site-specific …
CRISPR/Cas9 for genome editing: progress, implications and challenges
F Zhang, Y Wen, X Guo - Human molecular genetics, 2014 - academic.oup.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling …
(Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling …
Unravelling the structural and mechanistic basis of CRISPR–Cas systems
Bacteria and archaea have evolved sophisticated adaptive immune systems, known as
CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR …
CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR …
[PDF][PDF] CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity
R Barrangou, LA Marraffini - Molecular cell, 2014 - cell.com
Clustered regularly interspaced short palindromic repeats (CRISPR), and associated
proteins (Cas) comprise the CRISPR-Cas system, which confers adaptive immunity against …
proteins (Cas) comprise the CRISPR-Cas system, which confers adaptive immunity against …
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation
Introduction Bacteria and archaea defend themselves against invasive DNA using adaptive
immune systems comprising CRISPR (clustered regularly interspaced short palindromic …
immune systems comprising CRISPR (clustered regularly interspaced short palindromic …
Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity
The initial stage of CRISPR–Cas immunity involves the integration of foreign DNA spacer
segments into the host genomic CRISPR locus. The nucleases Cas1 and Cas2 are the only …
segments into the host genomic CRISPR locus. The nucleases Cas1 and Cas2 are the only …
Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes
MD Szczelkun, MS Tikhomirova… - Proceedings of the …, 2014 - National Acad Sciences
Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) systems protect bacteria and archaea from infection by viruses and plasmids. Central …
(Cas) systems protect bacteria and archaea from infection by viruses and plasmids. Central …
Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
F Zhang, RAN Fei - US Patent App. 14/104,837, 2014 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49883289&utm_source= google_patent&utm_medium= platform_link&utm_campaign …
49883289&utm_source= google_patent&utm_medium= platform_link&utm_campaign …
Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
F Zhang, RAN Fei - US Patent 8,865,406, 2014 - Google Patents
The invention provides for engineering and optimization of systems, methods, and
compositions for manipulation of sequences and/or activities of target sequences. Provided …
compositions for manipulation of sequences and/or activities of target sequences. Provided …
Target specificity of the CRISPR-Cas9 system
X Wu, AJ Kriz, PA Sharp - Quantitative biology, 2014 - Springer
The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been
repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome …
repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome …