As part of their life cycle, neutralophilic bacteria are often exposed to varying environmental
stresses, among which fluctuations in pH are the most frequent. In particular, acid …

Recent advances in quantitative single-cell analysis revealed large diversity in gene
expression levels between individual cells, which could affect the physiology and/or fate of …

Microbial rhodopsins are a diverse group of photoactive transmembrane proteins found in
all three domains of life. A member of this protein family, Archaerhodopsin-3 (Arch) of …

Methylglyoxal (MG) is a toxic by‐product of glycolysis that damages DNA and proteins
ultimately leading to cell death. Protection from MG is often conferred by a glutathione …

Abstract Background Inclusion bodies (IBs) are aggregated proteins that form clusters when
protein is overexpressed in heterologous expression systems. IBs have been considered as …

Measurement of intracellular pH can be readily accomplished using tools and methods
described in this chapter. We present a discussion of technical considerations of various …

Antimicrobial peptides are a class of compounds with broad-spectrum antibacterial activity,
found in several different species. While there has been considerable research on these …

Overnight Culture: Strains were incubated overnight in LBK buffered with 100mM 3-(N-
morpholino) propanesulfonic acid (MOPS) at pH 7.0 for standard lab conditions for …