Highlights•Addition of in vivo active aptamer domains for DNT and PPDA to the
toolbox.•Addition of expression platforms for transcriptional termination to the …

RNA utilizes many different mechanisms to control gene expression. Among the regulatory
elements that respond to external stimuli, riboswitches are a prominent and elegant …

Robust synthetic devices are requisite for the construction of synthetic genetic circuits and
important scientific and technological tools to control cellular processes. We developed …

Systems for conditional gene expression are powerful tools in basic research as well as in
biotechnology. For future applications, it is of great importance to engineer orthogonal …

In this review, we discuss new emerging medical applications of the rapidly evolving field of
mammalian synthetic biology. We start with simple mammalian synthetic biological …

Cytotoxicity of transgenes carried by adeno-associated virus (AAV) vectors might be desired,
for instance, in oncolytic virotherapy or occur unexpectedly in exploratory research when …

Highlights•Ribozyme activity can be regulated by chemical and physical stimuli.•Aptazymes
consist of RNA aptamers joined to ribozymes via a communication module.•Ribozymes can …

Antibody therapy of solid cancers is well established, but suffers from unsatisfactory tumor
penetration of large immunoglobulins or from low serum retention of antibody fragments …

The group I intron ribozyme from Tetrahymena was recently reengineered into a trans-
splicing variant that is able to remove 100-nt introns from pre-mRNA, analogous to the …

Synthetic RNA-based switches are a growing class of genetic controllers applied in synthetic
biology to engineer cellular functions. In this chapter, we detail a protocol for the selection of …