An updated evolutionary classification of CRISPR–Cas systems
KS Makarova, YI Wolf, OS Alkhnbashi… - Nature reviews …, 2015 - nature.com
The evolution of CRISPR–cas loci, which encode adaptive immune systems in archaea and
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
CRISPR-Cas immunity in prokaryotes
LA Marraffini - Nature, 2015 - nature.com
Prokaryotic organisms are threatened by a large array of viruses and have developed
numerous defence strategies. Among these, only clustered, regularly interspaced short …
numerous defence strategies. Among these, only clustered, regularly interspaced short …
[PDF][PDF] Discovery and functional characterization of diverse class 2 CRISPR-Cas systems
Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector
complexes, and Class 2, with single protein effectors. Currently, only two Class 2 effectors …
complexes, and Class 2, with single protein effectors. Currently, only two Class 2 effectors …
[PDF][PDF] A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants
CRISPR/Cas9 genome targeting systems have been applied to a variety of species.
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …
However, most CRISPR/Cas9 systems reported for plants can only modify one or a few …
[HTML][HTML] Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system
B Zetsche, JS Gootenberg, OO Abudayyeh… - cell, 2015 - cell.com
The microbial adaptive immune system CRISPR mediates defense against foreign genetic
elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize …
elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize …
Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA
S Svitashev, JK Young, C Schwartz, H Gao… - Plant …, 2015 - academic.oup.com
Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …
[HTML][HTML] Insights from 20 years of bacterial genome sequencing
Since the first two complete bacterial genome sequences were published in 1995, the
science of bacteria has dramatically changed. Using third-generation DNA sequencing, it is …
science of bacteria has dramatically changed. Using third-generation DNA sequencing, it is …
[HTML][HTML] The CRISPR-Cas immune system: biology, mechanisms and applications
Viruses are a common threat to cellular life, not the least to bacteria and archaea who
constitute the majority of life on Earth. Consequently, a variety of mechanisms to resist virus …
constitute the majority of life on Earth. Consequently, a variety of mechanisms to resist virus …
Annotation and classification of CRISPR-Cas systems
KS Makarova, EV Koonin - CRISPR: methods and protocols, 2015 - Springer
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (CRISPR-
associated proteins) is a prokaryotic adaptive immune system that is represented in most …
associated proteins) is a prokaryotic adaptive immune system that is represented in most …
Cas9 specifies functional viral targets during CRISPR–Cas adaptation
Clustered regularly interspaced short palindromic repeat (CRISPR) loci and their associated
(Cas) proteins provide adaptive immunity against viral infection in prokaryotes. Upon …
(Cas) proteins provide adaptive immunity against viral infection in prokaryotes. Upon …