Rapid PCR of STR markers: Applications to human identification

EL Romsos, PM Vallone - Forensic Science International: Genetics, 2015 - Elsevier
Multiplex PCR with fluorescently labeled primers has been an essential method for the
amplification of short tandem repeats used in human identify testing. Within the STR …

Rapid and controllable digital microfluidic heating by surface acoustic waves

RJ Shilton, V Mattoli, M Travagliati… - Advanced Functional …, 2015 - Wiley Online Library
Fast and controllable surface acoustic wave (SAW) driven digital microfluidic temperature
changes are demonstrated. Within typical operating conditions, the direct acoustic heating …

The rotary zone thermal cycler: a low-power system enabling automated rapid PCR

MS Bartsch, HS Edwards, D Lee, CE Moseley, KE Tew… - PloS one, 2015 - journals.plos.org
Advances in molecular biology, microfluidics, and laboratory automation continue to expand
the accessibility and applicability of these methods beyond the confines of conventional …

Development of a point-of-care-device for fast detection of periodontal pathogens

C Gaertig, K Niemann, J Berthold, L Giel, N Leitschuh… - BMC Oral Health, 2015 - Springer
Background A number of pathogens can cause severe destruction of the periodontal
apparatus during the course of periodontitis. The aim of this work was the development of a …

Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test

J Xu, X Lv, Y Wei, L Zhang, R Li, Y Deng… - Sensors and Actuators B …, 2015 - Elsevier
Due to the difficulty in sample introduction, the presence of air bubbles and the complicated
fabrication with high cost, there are no commercial products of microfluidic polymerase chain …

Assessment of surfactants for efficient droplet PCR in mineral oil using the pendant drop technique

KR Pandit, PE Rueger, RV Calabrese… - Colloids and Surfaces B …, 2015 - Elsevier
Amplification and detection of nucleic acid sequences within integrated microsystems is
routinely conducted using the technique of droplet PCR, wherein the polymerase chain …

[HTML][HTML] Nanoliter-sized overheated reactor

P Neužil, W Sun, T Karásek, A Manz - Applied Physics Letters, 2015 - pubs.aip.org
We report on a microfluidic system formed by 200 nl water droplets, encapsulated by a 600
nl mineral oil placed on a hydrophobically coated glass microscope cover slip. The …

Droplet actuator and droplet-based techniques

A Sudarsan, Z Hua - US Patent App. 14/493,729, 2015 - Google Patents
2015-08-27 Assigned to NATIONAL INSTITUTES OF HEALTH (NIH), US DEPT. OF HEALTH
AND HUMAN SERVICES (DHHS), US GOVERNMENT reassignment NATIONAL …

Rapid quantification of microRNAs in plasma using a fast real-time PCR system

WJ Andrews, ED Brown, M Dellett, RE Hogg… - …, 2015 - Taylor & Francis
The ability to rapidly detect circulating small RNAs, in particular microRNAs (miRNAs),
would further increase their already established potential as biomarkers for a range of …

Methods and compositions for rapid thermal cycling

NR Beer, WJ Benett, JM Frank, JR Deotte… - US Patent …, 2015 - Google Patents
The rapid thermal cycling of a material is targeted. A microf luidic heat exchanger with an
internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows …