Tandem shock waves in medicine and biology: a review of potential applications and successes

P Lukes, F Fernández, J Gutiérrez-Aceves… - Shock Waves, 2016 - Springer
Shock waves have been established as a safe and effective treatment for a wide range of
diseases. Research groups worldwide are working on improving shock wave technology …

Increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae by disruption of the genes encoding cell wall α-1,3-glucan …

K Miyazawa, A Yoshimi, S Zhang… - Bioscience …, 2016 - academic.oup.com
Under liquid culture conditions, the hyphae of filamentous fungi aggregate to form pellets,
which reduces cell density and fermentation productivity. Previously, we found that loss of α …

The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background

H Zhang, S Wang, XX Zhang, W Ji, F Song… - Microbial Cell …, 2016 - Springer
Abstract Background The filamentous fungus Aspergillus niger is widely exploited as an
important expression host for industrial production. The glucoamylase high-producing strain …

Alternative eukaryotic expression systems for the production of proteins and protein complexes

S Gómez, M López-Estepa, FJ Fernández… - … Technologies for Protein …, 2016 - Springer
Besides the most established expression hosts, several eukaryotic microorganisms and
filamentous fungi have also been successfully used as platforms for the production of foreign …

Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans

M Schalén, DC Anyaogu, JB Hoof… - Fungal Biology and …, 2016 - Springer
Background The considerable capacity of filamentous fungi for the secretion of proteins is
the basis for multi-billion dollar industries producing enzymes and proteins with therapeutic …

Structures of almond hydroxynitrile lyase isoenzyme 5 provide a rationale for the lack of oxidoreductase activity in flavin dependent HNLs

T Pavkov-Keller, J Bakhuis, G Steinkellner… - Journal of …, 2016 - Elsevier
Hydroxynitrile lyases (HNLs) catalyze the asymmetric addition of HCN to aldehydes
producing enantiomerically pure cyanohydrins. These enzymes can be heterologously …

Gene Expression Systems in Industrial Ascomycetes: Advancements and Applications

J Ramoni, V Seidl-Seiboth, RH Bischof… - Gene Expression Systems …, 2016 - Springer
Filamentous fungi have a long tradition as industrial producers of enzymes and other
proteins benefitting from their exceptional high capacity for the synthesis and secretion of …

Heterologous Protein Expression in the Aspergilli: Overcoming Obstacles in the Secretory Pathway

M Sanguinetti, A Ramón - Microbial Models: From Environmental to …, 2016 - Springer
Some Aspergillus species are widely used as cell factories for the production of
heterologous proteins, showing, among other advantages, an exceptional secretion …

[HTML][HTML] 米曲霉外源表达系统研究进展

于潇淳, 马世良 - 中国生物工程杂志, 2016 - html.rhhz.net
丝状真菌米曲霉是发酵工业的重要菌种, 具有强大的蛋白分泌能力和较高的食品安全性,
可作为表达外源蛋白的细胞工厂. 近年来, 米曲霉全基因组序列的测序完成和基于表达序列标签 …

粗糙脉孢菌蛋白表达系统构建研究

高染染, 刘倩, 孙文良, 孙志勇, 刘浩, 田朝光 - 生物技术通报, 2016 - biotech.aiijournal.com
旨在将模式丝状真菌粗糙脉孢菌(Neurospora crassa) 构建为蛋白质表达系统.
通过真菌杂交技术构建出粗糙脉孢菌六突变缺失菌株LQ-1 (Δ3βG∷ Δ2cbh∷ Δhis3) …