Hydrogenases catalyze the reversible conversion of molecular hydrogen to protons and
electrons via a heterolytic splitting mechanism. The active sites of [NiFe] hydrogenases …

In Escherichia coli, hydrogen metabolism plays a prominent role in anaerobic physiology.
The genome contains the capability to produce and assemble up to four [NiFe] …

H2/O2 biofuel cells utilizing hydrogenases and multicopper oxidases as bioelectrocatalysts
are clean, sustainable, and environmentally friendly power devices. In this study, we …

Abstract [NiFe] hydrogenases are metalloenzymes catalyzing the reversible heterolytic
cleavage of hydrogen into protons and electrons. Gas tunnels make the deeply buried active …

A novel group of bacterial [NiFe]-hydrogenases is responsible for high-affinity H 2 uptake
from the troposphere, and is therefore thought to play an important role in the global H 2 …

Abstract 'Oxygen-tolerant'[NiFe]-hydrogenases can catalyze H 2 oxidation under aerobic
conditions, avoiding oxygenation and destruction of the active site. In one mechanism …

Hydrogenases are enzymes of great biotechnological relevance because they catalyse the
interconversion of H2, water (protons) and electricity using non-precious metal catalytic …

Oxygen‐tolerant [NiFe] hydrogenases are attractive biocatalysts for utilization in H2/O2 fuel
cells, which thereby reduces the amount of platinum‐based catalysts. The O2‐tolerant …

The tolerance towards oxic conditions of O2-tolerant [NiFe] hydrogenases has been
attributed to an unusual [4Fe–3S] cluster that lies proximal to the [NiFe] active site. Upon …

Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved
catalytic bias towards hydrogen oxidation which limits their technological value. We present …