From dirt to industrial applications: Pseudomonas putida as a synthetic biology chassis for hosting harsh biochemical reactions
Highlights•SynBio needs chassis with superior genetic, biochemical, and physiological
traits.•Environmental bacteria are endowed with many advantageous metabolic properties.• …
traits.•Environmental bacteria are endowed with many advantageous metabolic properties.• …
Unknown unknowns: essential genes in quest for function
The experimental design of a minimal synthetic genome revealed the presence of a large
number of genes without ascribed function, in part because the abstract laws of life must be …
number of genes without ascribed function, in part because the abstract laws of life must be …
The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis
E Belda, RGA Van Heck… - Environmental …, 2016 - Wiley Online Library
By the time the complete genome sequence of the soil bacterium Pseudomonas putida
KT2440 was published in 2002 (Nelson et al.,) this bacterium was considered a potential …
KT2440 was published in 2002 (Nelson et al.,) this bacterium was considered a potential …
Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity
The conversion of biomass-derived sugars and aromatic molecules to cis, cis-muconic acid
(referred to hereafter as muconic acid or muconate) has been of recent interest owing to its …
(referred to hereafter as muconic acid or muconate) has been of recent interest owing to its …
Production of selenium nanoparticles in Pseudomonas putida KT2440
R Avendaño, N Chaves, P Fuentes, E Sánchez… - Scientific reports, 2016 - nature.com
Selenium (Se) is an essential element for the cell that has multiple applications in medicine
and technology; microorganisms play an important role in Se transformations in the …
and technology; microorganisms play an important role in Se transformations in the …
Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes
I Benedetti, V de Lorenzo, PI Nikel - Metabolic engineering, 2016 - Elsevier
Bacterial biofilms outperform planktonic counterparts in whole-cell biocatalysis. The
transition between planktonic and biofilm lifestyles of the platform strain Pseudomonas …
transition between planktonic and biofilm lifestyles of the platform strain Pseudomonas …
Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system
Background Pseudomonas putida is a promising host for the bioproduction of chemicals, but
its industrial applications are significantly limited by its obligate aerobic character. The aim of …
its industrial applications are significantly limited by its obligate aerobic character. The aim of …
The Ssr protein (T1E_1405) from Pseudomonas putida DOT‐T1E enables oligonucleotide‐based recombineering in platform strain P. putida EM42
T Aparicio, SI Jensen, AT Nielsen… - Biotechnology …, 2016 - Wiley Online Library
Some strains of the soil bacterium Pseudomonas putida have become in recent years
platforms of choice for hosting biotransformations of industrial interest. Despite availability of …
platforms of choice for hosting biotransformations of industrial interest. Despite availability of …
The quest for the minimal bacterial genome
E Martínez-García, V de Lorenzo - Current Opinion in Biotechnology, 2016 - Elsevier
Highlights•The quest for minimal genomes has both fundamental and biotechnological
motivations.•Genome reduction has been enabled by chromosomal editing and chemical …
motivations.•Genome reduction has been enabled by chromosomal editing and chemical …
Metabolic Engineering of Pseudomonas putida KT2440 for the Production of para-Hydroxy Benzoic Acid
para-Hydroxy benzoic acid (PHBA) is the key component for preparing parabens, a common
preservatives in food, drugs, and personal care products, as well as high-performance …
preservatives in food, drugs, and personal care products, as well as high-performance …