Chromatin, the template for epigenetic regulation, is a highly dynamic entity that is constantly
reshaped during early development and differentiation. Epigenetic modification of chromatin …

The regulative capability of single cells to give rise to all primary embryonic lineages is
termed pluripotency. Observations of fluctuating gene expression and phenotypic …

Interspecies blastocyst complementation enables organ-specific enrichment of xenogenic
pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst …

Of all known cultured stem cell types, pluripotent stem cells (PSCs) sit atop the landscape of
developmental potency and are characterized by their ability to generate all cell types of an …

Chromatin looping is key to gene regulation, yet no broadly applicable methods to
selectively modify chromatin loops have been described. We have engineered a method for …

Much attention has focussed on the conversion of human pluripotent stem cells (PSCs) to a
more naïve developmental status. Here we provide a method for resetting via transient …

Concomitant activation of the Wnt pathway and suppression of Mapk signalling by two small
molecule inhibitors (2i) in the presence of leukaemia inhibitory factor (LIF)(hereafter termed …

Pathways underlying mouse embryonic development have always informed efforts to derive,
maintain, and drive differentiation of human pluripotent stem cells. However, direct …

The foundations of mammalian development lie in a cluster of embryonic epiblast stem cells.
In response to extracellular matrix signalling, these cells undergo epithelialization and …

Variability in induced pluripotent stem cell (iPSC) lines remains a concern for disease
modeling and regenerative medicine. We have used RNA-sequencing analysis and linear …