Maximizing the stability of metabolic engineering‐derived whole‐cell biocatalysts

M Kadisch, C Willrodt, M Hillen, B Bühler… - Biotechnology …, 2017 - Wiley Online Library
A systematic and powerful knowledge‐based framework exists for improving the activity and
stability of chemical catalysts and for empowering the commercialization of respective …

Chemoenzymatic epoxidation of alkenes with Candida antarctica lipase B and hydrogen peroxide in deep eutectic solvents

P Zhou, X Wang, B Yang, F Hollmann, Y Wang - RSC advances, 2017 - pubs.rsc.org
Epoxides are important synthetic intermediates for the synthesis of a broad range of
industrial products. This study presents a promising solution to the current limitation of …

Maximization of cell viability rather than biocatalyst activity improves whole‐cell ω‐oxyfunctionalization performance

M Kadisch, MK Julsing, M Schrewe… - Biotechnology and …, 2017 - Wiley Online Library
It is a common misconception in whole‐cell biocatalysis to refer to an enzyme as the
biocatalyst, thereby neglecting the structural and metabolic framework provided by the cell …

Enhancing productivity for cascade biotransformation of styrene to (S)-vicinal diol with biphasic system in hollow fiber membrane bioreactor

P Gao, S Wu, P Praveen, KC Loh, Z Li - Applied microbiology and …, 2017 - Springer
Biotransformation is a green and useful tool for sustainable and selective chemical
synthesis. However, it often suffers from the toxicity and inhibition from organic substrates or …

The application of constitutively solvent‐tolerant P. taiwanensis VLB120ΔCΔttgV for stereospecific epoxidation of toxic styrene alleviates carrier solvent use

J Volmer, A Schmid, B Bühler - Biotechnology Journal, 2017 - Wiley Online Library
For whole‐cell biotransformations involving toxic organic compounds, two‐liquid phase
setups are typically applied employing an apolar extractive phase. Bis (2‐ethylhexyl) …

Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing

M Kadisch, A Schmid, B Bühler - Journal of Industrial …, 2017 - academic.oup.com
Fatty acid methyl esters (FAMEs) originating from plant oils are most interesting renewable
feedstocks for biofuels and bio-based materials. FAMEs can also be produced and/or …