Capillary electrophoresis–mass spectrometry for direct structural identification of serum N-glycans

CM Snyder, X Zhou, JA Karty, BR Fonslow… - … of chromatography A, 2017 - Elsevier
Through direct coupling of capillary electrophoresis (CE) to mass spectrometry (MS) with a
sheathless interface, we have identified 77 potential N-glycan structures derived from …

Automated N-glycosylation sequencing of biopharmaceuticals by capillary electrophoresis

M Szigeti, A Guttman - Scientific reports, 2017 - nature.com
Comprehensive analysis of the N-linked carbohydrates of glycoproteins is gaining high
recent interest in both the biopharmaceutical and biomedical fields. In addition to high …

Microscale measurements of Michaelis–Menten constants of neuraminidase with nanogel capillary electrophoresis for the determination of the sialic acid linkage

S Gattu, CL Crihfield, LA Holland - Analytical chemistry, 2017 - ACS Publications
Phospholipid nanogels enhance the stability and performance of the exoglycosidase
enzyme neuraminidase and are used to create a fixed zone of enzyme within a capillary …

Plug-plug kinetic capillary electrophoresis for in-capillary exoglycosidase digestion as a profiling tool for the analysis of glycoprotein glycans

M Yamagami, Y Matsui, T Hayakawa… - … of Chromatography A, 2017 - Elsevier
An online exoglycosidase digestion was combined with a plug-plug kinetic mode of capillary
electrophoresis (CE) for the analysis of glycoprotein-derived oligosaccharides. An …

Capillary electrophoresis with stationary nanogel zones of galactosidase and Erythrina cristagalli lectin for the determination of β (1–3)-linked galactose in glycans

LA Holland, S Gattu, CL Crihfield, L Bwanali - Journal of Chromatography A, 2017 - Elsevier
A thermally responsive nanogel is used to create stationary zones of enzyme and lectin in a
separation capillary. Once patterned in the capillary, analyte is driven through the zone …

Carbohydrate analysis on hybrid poly (dimethylsiloxane)/glass chips dynamically coated with ionic complementary peptide

N Li, X Hai, X Yu, F Dang - Journal of Chromatography A, 2017 - Elsevier
A facile and efficient dynamic coating method using an ionic complementary peptide was
established for high-performance separation of 8-aminopyrene-1, 3, 6-trisulfonic acid …