Use of monolithic supports for highthroughput protein and peptide separation in proteomics

U Andjelković, S Tufegdžić, M Popović - Electrophoresis, 2017 - Wiley Online Library
The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low
back pressure, easy preparation process and miniaturisation, and the availability of different …

Comparative evaluation of labelfree quantification methods for shotgun proteomics

JA Bubis, LI Levitsky, MV Ivanov… - Rapid …, 2017 - Wiley Online Library
Rationale Labelfree quantification (LFQ) is a popular strategy for shotgun proteomics. A
variety of LFQ algorithms have been developed recently. However, a comprehensive …

[HTML][HTML] Sex-specific biology of the human malaria parasite revealed from the proteomes of mature male and female gametocytes

J Miao, Z Chen, Z Wang, S Shrestha, X Li, R Li… - Molecular & Cellular …, 2017 - ASBMB
The gametocytes of the malaria parasites are obligate for perpetuating the parasite's life
cycle through mosquitoes, but the sex-specific biology of gametocytes is poorly understood …

Mycobacterium tuberculosis Infection Manipulates the Glycosylation Machinery and the N-Glycoproteome of Human Macrophages and Their Microparticles

NJ Hare, LY Lee, I Loke, WJ Britton… - Journal of proteome …, 2017 - ACS Publications
Tuberculosis (TB) remains a prevalent and lethal infectious disease. The glycobiology
associated with Mycobacterium tuberculosis infection of frontline alveolar macrophages is …

[HTML][HTML] Differential protein expression in chicken macrophages and heterophils in vivo following infection with Salmonella Enteritidis

Z Sekelova, H Stepanova, O Polansky, K Varmuzova… - Veterinary …, 2017 - Springer
In this study we compared the proteomes of macrophages and heterophils isolated from the
spleen 4 days after intravenous infection of chickens with Salmonella Enteritidis. Heterophils …

ABRF Proteome Informatics Research Group (iPRG) 2015 Study: detection of differentially abundant proteins in label-free quantitative LC–MS/MS experiments

M Choi, ZF Eren-Dogu, C Colangelo… - Journal of proteome …, 2017 - ACS Publications
Detection of differentially abundant proteins in label-free quantitative shotgun liquid
chromatography–tandem mass spectrometry (LC–MS/MS) experiments requires a series of …

Physiological and proteomic characterization of light adaptations in marine Synechococcus

KRM Mackey, AF Post, MR McIlvin… - Environmental …, 2017 - Wiley Online Library
Marine Synechococcus thrive over a range of light regimes in the ocean. We examined the
proteomic, genomic and physiological responses of seven Synechococcus isolates to …

Ricin-like proteins from the castor plant do not influence liquid chromatography-mass spectrometry detection of ricin in forensically relevant samples

ED Merkley, SC Jenson, JS Arce, AM Melville… - Toxicon, 2017 - Elsevier
The toxic protein ricin (also known as RCA60), found in the seed of the castor plant (Ricinus
communis) is frequently encountered in law enforcement investigations. The ability to detect …

Multiple marker abundance profiling: combining selected reaction monitoring and datadependent acquisition for rapid estimation of organelle abundance in …

CM Hooper, TJ Stevens, A Saukkonen… - The Plant …, 2017 - Wiley Online Library
Measuring changes in protein or organelle abundance in the cell is an essential, but
challenging aspect of cell biology. Frequentlyused methods for determining organelle …

Poly (Nacryloxysuccinimidecoethylene glycol dimethacrylate) precursor monolith and its post polymerization modification with alkyl ligands, trypsin and lectins for …

M Jonnada, Z El Rassi - Electrophoresis, 2017 - Wiley Online Library
This investigation was aimed at introducing a monolithic precursor that can be conveniently
grafted with the desired chromatographic ligand via the process of post polymerization …