Because of its safe and effective protein expression profile, in vitro transcribed messenger
RNA (IVT-mRNA) represents a promising candidate in the development of novel …

Sleeping Beauty (SB) is the first synthetic DNA transposon that was shown to be active in a
wide variety of species. Here, we review studies from the last two decades addressing both …

CRISPR-Cas9 technology has accelerated biological research becoming routine for many
laboratories. It is rapidly replacing conventional gene editing techniques and has high utility …

The history of transgenesis is marked by milestones such as the development of cellular
transdifferentiation, recombinant DNA, genetic modification of target cells, and finally, the …

Foot and mouth disease, which is induced by the foot and mouth disease virus (FMDV),
takes its toll on the cloven-hoofed domestic animals. The VP1 gene in FMDV genome …

Transgenic domestic animals represent an alternative to bioreactors for large-scale
production of biopharmaceuticals and could also provide more accurate biomedical models …

Results VP1-shRNA inhibits the expression of FMDV-VP1. The FMDV-VP1 sequences were
analyzed, and the shRNA sequences were screened and synthesized. After annealing, the …