High throughput genome sequencing has revealed a multitude of potential secondary
metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with …

CRISPR-Cas9 technologies are revolutionizing fungal gene editing. Here we show that
survival of specific Cas9/sgRNA mediated DNA double strand breaks (DSBs) depends on …

The present work describes the application of homologous recombination techniques in a
wild-type Aspergillus terreus (ATCC 20542) strain to increase the flow of precursors towards …

Tannin acyl hydrolase (tannase, EC3. 1.1. 20) catalyzes the hydrolysis of hydrolyzable
tannins. It is used in the manufacture of instant tea and in the production of gallic acid. In this …

Efficient protein secretion is often a valuable alternative to classic cellular expression to
obtain homogenous protein samples. Early on, bacterial type I secretion systems (T1SS) …

Acidic pectin lyase is of great application value in fruit-processing industry. In this study, five
pectin lyase genes (pelA, pelB, pelC, pelD and pelF) from A. niger pectin lyase gene family …

Filamentous fungi are lower eukaryotes increasingly used for expression of foreign proteins
ranging from industrial enzymes originating from other fungi and bacteria to proteins of …

In this study, we characterised PgpdA, PgpdA2B, PgpdA3B and PgpdA4B promoters,
containing 1–4 copies of gpd box by modifying the gpdA promoter, and constructed pSZHGX …

丝状真菌米曲霉(Aspergillus oryzae) 是发酵工业的重要菌株, 具有强大的蛋白分泌能力和公认
的食品安全性, 可作为表达外源蛋白的细胞工厂. 然而利用米曲霉分泌生产外源蛋白质常会受限 …

The filamentous fungus Aspergillus oryzae is an important strain in fermentation industry. It
has a strong capacity of secreting protein and recognized food safety, therefore is widely …