Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways
Here we develop a methylation editing toolbox, Casilio-ME, that enables not only RNA-
guided methylcytosine editing by targeting TET1 to genomic sites, but also by co-delivering …
guided methylcytosine editing by targeting TET1 to genomic sites, but also by co-delivering …
Excision of 5-carboxylcytosine by thymine DNA glycosylase
5-Methylcytosine (mC) is an epigenetic mark that is written by methyltransferases, erased
through passive and active mechanisms, and impacts transcription, development, diseases …
through passive and active mechanisms, and impacts transcription, development, diseases …
Thymine DNA glycosylase recognizes the geometry alteration of minor grooves induced by 5-formylcytosine and 5-carboxylcytosine
T Fu, L Liu, QL Yang, Y Wang, P Xu, L Zhang, S Liu… - Chemical …, 2019 - pubs.rsc.org
The dynamic DNA methylation–demethylation process plays critical roles in gene
expression control and cell development. The oxidation derivatives of 5-methylcytosine …
expression control and cell development. The oxidation derivatives of 5-methylcytosine …
Label-free and amplified electrogenerated chemiluminescence biosensing for the detection of thymine DNA glycosylase activity using DNA-functionalized gold …
W Bai, Y Wei, Y Zhang, L Bao, Y Li - Analytica Chimica Acta, 2019 - Elsevier
Effective detection of thymine DNA glycosylase (TDG) activity is extremely crucial and urgent
for epigenetic research. Herein, a novel label-free electrogenerated chemiluminescence …
for epigenetic research. Herein, a novel label-free electrogenerated chemiluminescence …
5-Carboxylcytosine and cytosine protonation distinctly alter the stability and dehybridization dynamics of the DNA duplex
Applications associated with nucleobase protonation events are grounded in their
fundamental impact on DNA thermodynamics, structure, and hybridization dynamics. Of the …
fundamental impact on DNA thermodynamics, structure, and hybridization dynamics. Of the …
Thymine DNA glycosylase is a key regulator of CaMKIIγ expression and vascular smooth muscle phenotype
YF Liu, LY Sun, DV Singer, R Ginnan… - American Journal …, 2019 - journals.physiology.org
Multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a multigene family
with isoform-specific regulation of vascular smooth muscle (VSM) functions. In previous …
with isoform-specific regulation of vascular smooth muscle (VSM) functions. In previous …
The role of active-site residues Phe98, HiS239, and Arg243 in DNA binding and in the catalysis of human uracil–DNA glycosylase SMUG1
DA Iakovlev, IV Alekseeva, YN Vorobjev, NA Kuznetsov… - Molecules, 2019 - mdpi.com
Human SMUG1 (hSMUG1) hydrolyzes the N-glycosidic bond of uracil and some uracil
lesions formed in the course of epigenetic regulation. Despite the functional importance of …
lesions formed in the course of epigenetic regulation. Despite the functional importance of …
Petri net–based model of the human DNA base excision repair pathway
Cellular DNA is daily exposed to several damaging agents causing a plethora of DNA
lesions. As a first aid to restore DNA integrity, several enzymes got specialized in damage …
lesions. As a first aid to restore DNA integrity, several enzymes got specialized in damage …
Casilio-ME: Enhanced CRISPR-based DNA demethylation by RNA-guided coupling methylcytosine oxidation and DNA repair pathways
We have developed a methylation editing toolbox, Casilio-ME, that enables not only RNA-
guided methylcytosine editing by targeting TET1 to genomic sites, but also by co-delivering …
guided methylcytosine editing by targeting TET1 to genomic sites, but also by co-delivering …
[HTML][HTML] Chromatin and Epigenetics in Cardiovascular Disease: Thymine DNA glycosylase is a key regulator of CaMKIIγ expression and vascular smooth muscle …
YF Liu, LY Sun, DV Singer, R Ginnan… - American Journal of …, 2019 - ncbi.nlm.nih.gov
Abstract Multifunctional Ca 2+/calmodulin-dependent protein kinase II (CaMKII) is a
multigene family with isoform-specific regulation of vascular smooth muscle (VSM) functions …
multigene family with isoform-specific regulation of vascular smooth muscle (VSM) functions …