The virome is comprised of endogenous retroviruses, eukaryotic viruses, and
bacteriophages and is increasingly being recognized as an essential part of the human …

Next generation sequencing (NGS) technologies provide an increasingly important avenue
for detecting known viruses, and for discovering novel viruses present in clinical or …

Human interaction with animals has been implicated as a primary risk factor for several high
impact zoonoses, including many bat-origin viral diseases. However the animal-to-human …

Objectives Sample preparation for high-throughput sequencing (HTS) includes treatment
with various laboratory components, potentially carrying viral nucleic acids, the extent of …

Objectives Travel-associated infections are challenging to diagnose because of the broad
spectrum of potential aetiologies. As a proof-of-principle study, we used MNGS to identify …

Quality management and independent assessment of high-throughput sequencing-based
virus diagnostics have not yet been established as a mandatory approach for ensuring …

The frequency of reported outbreaks of infectious diseases has increased over the past 3
decades, with predictions that this rise will continue. Outbreak response continues to follow …

We previously demonstrated that shotgun metagenomic sequencing can detect bacteria in
sonicate fluid, providing a diagnosis of prosthetic joint infection (PJI). A limitation of the …

In recent years, it has become evident that a generational gap has developed in the
community of arbovirus research. This apparent gap is due to the dis-investment of training …

BACKGROUND Next-generation sequencing (NGS) is revolutionizing a variety of molecular
biology fields including bioforensics, biosurveillance, and infectious disease diagnostics. For …