CRISPR/Cas systems towards next-generation biosensing

Y Li, S Li, J Wang, G Liu - Trends in biotechnology, 2019 - cell.com
Beyond its remarkable genome editing ability, the CRISPR/Cas9 effector has also been
utilized in biosensing applications. The recent discovery of the collateral RNA cleavage …

Principles and applications of nucleic acid strand displacement reactions

FC Simmel, B Yurke, HR Singh - Chemical reviews, 2019 - ACS Publications
Dynamic DNA nanotechnology, a subfield of DNA nanotechnology, is concerned with the
study and application of nucleic acid strand-displacement reactions. Strand-displacement …

Origins and evolution of CRISPR-Cas systems

EV Koonin, KS Makarova - Philosophical Transactions of …, 2019 - royalsocietypublishing.org
CRISPR-Cas, the bacterial and archaeal adaptive immunity systems, encompass a complex
machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic …

[HTML][HTML] Methodologies for improving HDR efficiency

M Liu, S Rehman, X Tang, K Gu, Q Fan, D Chen… - Frontiers in …, 2019 - frontiersin.org
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9
(Cas9) is a precise genome manipulating technology that can be programmed to induce …

Molecular mechanisms of RNA targeting by Cas13-containing type VI CRISPR–Cas systems

MR O'Connell - Journal of molecular biology, 2019 - Elsevier
Prokaryotic adaptive immune systems use Clustered Regularly Interspaced Short
Palindromic Repeats (CRISPRs) and CRISPR-associated (Cas) proteins for RNA-guided …

Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription

JA Doudna, M Jinek, E Charpentier… - US Patent …, 2019 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49624232&utm_source= google_patent&utm_medium= platform_link&utm_campaign …

[HTML][HTML] CRISPR-Cas13d mediates robust RNA virus interference in plants

A Mahas, R Aman, M Mahfouz - Genome biology, 2019 - Springer
Background CRISPR-Cas systems endow bacterial and archaeal species with adaptive
immunity mechanisms to fend off invading phages and foreign genetic elements. CRISPR …

[HTML][HTML] Plant genome engineering for targeted improvement of crop traits

KEM Sedeek, A Mahas, M Mahfouz - Frontiers in plant science, 2019 - frontiersin.org
To improve food security, plant biology research aims to improve crop yield and tolerance to
biotic and abiotic stress, as well as increasing the nutrient contents of food. Conventional …

CRISPR ethics: moral considerations for applications of a powerful tool

C Brokowski, M Adli - Journal of molecular biology, 2019 - Elsevier
With the emergence of CRISPR technology, targeted editing of a wide variety of genomes is
no longer an abstract hypothetical, but occurs regularly. As application areas of CRISPR are …

[HTML][HTML] Mutagenesis in rice: the basis for breeding a new super plant

VE Viana, C Pegoraro, C Busanello… - Frontiers in plant …, 2019 - frontiersin.org
The high selection pressure applied in rice breeding since its domestication thousands of
years ago has caused a narrowing in its genetic variability. Obtaining new rice cultivars …