LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification

H Zhang, Y Xu, Z Fohlerova, H Chang, C Iliescu… - TrAC Trends in …, 2019 - Elsevier
Nucleic acid amplification for the detection of infectious diseases, food pathogens, or
assessment of genetic disorders require a laboratory setting with specialized equipment and …

Emerging ultrafast nucleic acid amplification technologies for next-generation molecular diagnostics

SH Lee, S Park, BN Kim, OS Kwon, WY Rho… - Biosensors and …, 2019 - Elsevier
Over the last decade, nucleic acid amplification tests (NAATs) including polymerase chain
reaction (PCR) were an indispensable methodology for diagnosing cancers, viral and …

SHERLOCK: nucleic acid detection with CRISPR nucleases

MJ Kellner, JG Koob, JS Gootenberg, OO Abudayyeh… - Nature protocols, 2019 - nature.com
Rapid detection of nucleic acids is integral to applications in clinical diagnostics and
biotechnology. We have recently established a CRISPR-based diagnostic platform that …

Revealing the secrets of PCR

H Zhang, H Li, H Zhu, J Pekárek, P Podešva… - Sensors and Actuators B …, 2019 - Elsevier
The polymerase chain reaction (PCR) method and its quantitative variant (qPCR) were a
landmark discovery for detection and quantification of small amounts of unambiguous …

A fully portable microchip real‐time polymerase chain reaction for rapid detection of pathogen

R Tong, L Zhang, Q Song, C Hu, X Chen, K Lou… - …, 2019 - Wiley Online Library
Point‐of‐care detection for pathogen is of critical need for wide epidemic warning and
medical diagnosis. In this work, we have designed and developed a fully portable and …

Ultra-fast, sensitive and quantitative on-chip detection of group B streptococci in clinical samples

Q Cai, M Fauvart, RS Wiederkehr, B Jones, P Cools… - Talanta, 2019 - Elsevier
PCR enables sensitive and specific detection of infectious disease agents, but application in
point-of-care diagnostic testing remains scarce. A compact tool that runs PCR assays in less …

[HTML][HTML] Polymerase chain reaction using “V” shape thermal cycling program

R Chen, X Lu, M Li, G Chen, Y Deng, F Du, J Dong… - Theranostics, 2019 - ncbi.nlm.nih.gov
Polymerase chain reaction (PCR) is the most commonly used technique in molecular
biology and diagnostics. To achieve faster PCR reaction time, two strategies were employed …

Low-cost and disposable electrowetting-on-dielectric lab on a chip with an integrated electrochemical detector fabricated by screen-printing process

K Ugsornrat, P Pasakon, C Karuwan… - IEEE Sensors …, 2019 - ieeexplore.ieee.org
In this paper, a low-cost and disposable electrowetting-on-dielectric (EWOD) digital
microfluidic chip with an integrated electrochemical detector was fabricated based on a …

Small-Size Polymerase Chain Reaction Device with Improved Heat Transfer and Combined Feedforward/Feedback Control Strategy

M Gregorini, G Mikutis, RN Grass… - Industrial & Engineering …, 2019 - ACS Publications
We report improvements on the heat transfer and on the control strategy of a thermal cycler
implemented on a novel device for performing a fast polymerase chain reaction (PCR). The …

運用奈米狹縫表面電漿共振晶片結合微流道聚合酶連鎖反應裝置用於鼻咽癌DNA 檢測

阮柏翰 - 2019 - tdr.lib.ntu.edu.tw
表面電漿共振晶片常作為生物感測器, 因其具有即時量測, 免標定分析等特性,
同時利用自動化奈米壓印技術能夠低成本且大量製造表面電漿共振晶片, 非常適合應用於檢測 …