Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
The number and diversity of known CRISPR–Cas systems have substantially increased in
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …
The arms race between bacteria and their phage foes
HG Hampton, BNJ Watson, PC Fineran - Nature, 2020 - nature.com
Bacteria are under immense evolutionary pressure from their viral invaders—
bacteriophages. Bacteria have evolved numerous immune mechanisms, both innate and …
bacteriophages. Bacteria have evolved numerous immune mechanisms, both innate and …
Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay
The recent outbreak of novel coronavirus (SARS-CoV-2) causing COVID-19 disease
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …
Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2
The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for
controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of …
controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of …
[HTML][HTML] Massively multiplexed nucleic acid detection with Cas13
The great majority of globally circulating pathogens go undetected, undermining patient care
and hindering outbreak preparedness and response. To enable routine surveillance and …
and hindering outbreak preparedness and response. To enable routine surveillance and …
How to measure and evaluate binding affinities
I Jarmoskaite, I AlSadhan, PP Vaidyanathan… - Elife, 2020 - elifesciences.org
Quantitative measurements of biomolecule associations are central to biological
understanding and are needed to build and test predictive and mechanistic models. Given …
understanding and are needed to build and test predictive and mechanistic models. Given …
Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities
JE van Dongen, JTW Berendsen… - Biosensors and …, 2020 - Elsevier
With the trend of moving molecular tests from clinical laboratories to on-site testing, there is a
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
An ultralocalized Cas13a assay enables universal and nucleic acid amplification-free single-molecule RNA diagnostics
T Tian, B Shu, Y Jiang, M Ye, L Liu, Z Guo, Z Han… - ACS …, 2020 - ACS Publications
Existing methods for RNA diagnostics, such as reverse transcription PCR (RT-PCR), mainly
rely on nucleic acid amplification (NAA) and RT processes, which are known to introduce …
rely on nucleic acid amplification (NAA) and RT processes, which are known to introduce …
[HTML][HTML] iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2
The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life.
Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are …
Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are …
Massively parallel Cas13 screens reveal principles for guide RNA design
Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable
specific and robust target gene knockdown without altering the genome. To define rules for …
specific and robust target gene knockdown without altering the genome. To define rules for …