[HTML][HTML] Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species

I Poblete‐Castro, C Wittmann… - Microbial …, 2020 - Wiley Online Library
The use of renewable waste feedstocks is an environment‐friendly choice contributing to the
reduction of waste treatment costs and increasing the economic value of industrial by …

[HTML][HTML] A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida

P Calero, DC Volke, PT Lowe, CH Gotfredsen… - Nature …, 2020 - nature.com
Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture
and materials. Addition of fluorine to organic structures represents a unique strategy for …

[HTML][HTML] Engineered Pseudomonas putida simultaneously catabolizes five major components of corn stover lignocellulose: Glucose, xylose, arabinose, p-coumaric …

JR Elmore, GN Dexter, D Salvachúa, M O'Brien… - Metabolic …, 2020 - Elsevier
Valorization of all major lignocellulose components, including lignin, cellulose, and
hemicellulose is critical for an economically viable bioeconomy. In most biochemical …

Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440

GJ Bentley, N Narayanan, RK Jha, D Salvachúa… - Metabolic …, 2020 - Elsevier
Pseudomonas putida KT2440 has received increasing attention as an important biocatalyst
for the conversion of diverse carbon sources to multiple products, including the olefinic …

Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection

NT Wirth, E Kozaeva, PI Nikel - Microbial Biotechnology, 2020 - Wiley Online Library
Pseudomonas species have become reliable platforms for bioproduction due to their
capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their …

Fatty acid and alcohol metabolism in Pseudomonas putida: functional analysis using random barcode transposon sequencing

MG Thompson, MR Incha, AN Pearson… - Applied and …, 2020 - Am Soc Microbiol
With its ability to catabolize a wide variety of carbon sources and a growing engineering
toolkit, Pseudomonas putida KT2440 is emerging as an important chassis organism for …

[HTML][HTML] Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida

DC Volke, L Friis, NT Wirth, J Turlin, PI Nikel - Metabolic engineering …, 2020 - Elsevier
Genome engineering of non-conventional microorganisms calls for the development of
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …

Contextual flexibility in Pseudomonas aeruginosa central carbon metabolism during growth in single carbon sources

SK Dolan, M Kohlstedt, S Trigg, P Vallejo Ramirez… - MBio, 2020 - Am Soc Microbiol
Pseudomonas aeruginosa is an opportunistic human pathogen, particularly noted for
causing infections in the lungs of people with cystic fibrosis (CF). Previous studies have …

Pseudomonas as Versatile Aromatics Cell Factory

T Schwanemann, M Otto, N Wierckx… - Biotechnology …, 2020 - Wiley Online Library
Aromatics and their derivatives are valuable chemicals with a plethora of important
applications and thus play an integral role in modern society. Their current production relies …