Next generation sequencing and bioinformatics methodologies for infectious disease research and public health: approaches, applications, and considerations for …

I Maljkovic Berry, MC Melendrez… - The Journal of …, 2020 - academic.oup.com
Next generation sequencing (NGS) combined with bioinformatics has successfully been
used in a vast array of analyses for infectious disease research of public health relevance …

From the pipeline to the bedside: advances and challenges in clinical metagenomics

A Dulanto Chiang, JP Dekker - The Journal of infectious …, 2020 - academic.oup.com
Next-generation sequencing (NGS) technologies have revolutionized multiple areas in the
field of infectious diseases, from pathogen discovery to characterization of genes mediating …

Self-disseminating vaccines to suppress zoonoses

SL Nuismer, JJ Bull - Nature Ecology & Evolution, 2020 - nature.com
The SARS-CoV-2 epidemic is merely the most recent demonstration that our current
approach to emerging zoonotic infectious disease is ineffective. SARS, MERS, Ebola, Nipah …

Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus

E Fahsbender, A Charlys da-Costa, D Elise Gill… - PLoS …, 2020 - journals.plos.org
Plasma from patients with dengue-like symptoms was collected in 2013 to 2016 from the
Brazilian states of Tocantins and Amapa. 781 samples testing negative for IgM against …

Genome reconstruction of white spot syndrome virus (WSSV) from archival Davidson's-fixed paraffin embedded shrimp (Penaeus vannamei) tissue

R Cruz-Flores, HN Mai, S Kanrar… - Scientific Reports, 2020 - nature.com
Formalin-fixed paraffin-embedded (FFPE) tissues are a priceless resource for diagnostic
laboratories worldwide. However, DNA extracted from these tissues is often not optimal for …

MegaPath: sensitive and rapid pathogen detection using metagenomic NGS data

CM Leung, D Li, Y Xin, WC Law, Y Zhang, HF Ting… - BMC genomics, 2020 - Springer
Background Next-generation sequencing (NGS) enables unbiased detection of pathogens
by mapping the sequencing reads of a patient sample to the known reference sequence of …

A lateral flow biosensor based on gold nanoparticles detects four hemorrhagic fever viruses

J Zhao, S Fang, Y Liu, L Zeng, Z He - Analytical Methods, 2020 - pubs.rsc.org
The pathogen of viral hemorrhagic fever (VHF), which is harmful to human health, is a
hemorrhagic fever virus. Clinicians have long needed convenient and sensitive point-of-care …

Human tibroviruses: Commensals or lethal pathogens?

JH Kuhn, H Pān, CY Chiu, M Stremlau - Viruses, 2020 - mdpi.com
Rhabdoviruses are a large and ecologically diverse family of negative-sense RNA viruses
(Mononegavirales: Rhabdoviridae). These viruses are capable of infecting an unexpectedly …

The utility of human immune system mice for high-containment viral hemorrhagic fever research

DM Wozniak, KJ Lavender, J Prescott, JR Spengler - Vaccines, 2020 - mdpi.com
Human immune system (HIS) mice are a subset of humanized mice that are generated by
xenoengraftment of human immune cells or tissues and/or their progenitors into …

[PDF][PDF] Charlys da-Costa A, Elise Gill D, Augusto de Padua Milagres F, Brustulin R, Julio Costa Monteiro F. et al. 2020. Plasma virome of 781 Brazilians with …

E Fahsbender - PLoS One, 2020 - observatorio.fm.usp.br
Plasma from patients with dengue-like symptoms was collected in 2013 to 2016 from the
Brazilian states of Tocantins and Amapa. 781 samples testing negative for IgM against …