Continuous directed evolution methods allow the key steps of evolution—gene
diversification, selection, and replication—to proceed in the laboratory with minimal …

Success in synthetic biology and metabolic engineering is quickly becoming 'test'limited
within the design–build–test cycle. Commonly used methods for high-throughput screening …

In vivo mutagenesis systems accelerate directed protein evolution but often show restricted
capabilities and deleterious off-site mutations on cells. To overcome these limitations, here …

We present automated continuous evolution (ACE), a platform for the hands-free directed
evolution of biomolecules. ACE pairs OrthoRep, a genetic system for continuous targeted …

New technologies to target nucleotide diversification in vivo are promising enabling
strategies to perform directed evolution for engineering applications and forward genetics for …

Antibiotic biosynthetic gene clusters (BGCs) produce bioactive metabolites that impart a
fitness advantage to their producer, providing a mechanism for natural selection. This …

Highlights•Protein engineering is a powerful tool to generate proteins with desired
functions.•Computational and in vivo protein design opens new avenues for protein …

In vivo biosensors are powerful tools for metabolic engineering and synthetic biology
applications. However, the development of biosensors is hindered by the limited number of …

Through iterative rounds of genetic diversification and screening or selection, directed
evolution has been widely used to engineer relatively simple biosystems such as nucleic …

Synthetic biology provides powerful tools and novel strategies for designing and modifying
microorganisms to function as cell factories for biomanufacturing, which is a promising …