New advances in genetic engineering for L-theanine biosynthesis

SH Liu, J Li, JA Huang, ZH Liu, LG Xiong - Trends in Food Science & …, 2021 - Elsevier
Background l-theanine, the most represented non-proteinogenic amino acid in tea plants
(Camellia sinensis), is extensively utilized as a food supplement and a pharmaceutical …

Shading Promoted Theanine Biosynthesis in the Roots and Allocation in the Shoots of the Tea Plant (Camellia sinensis L.) Cultivar Shuchazao

T Yang, Y Xie, X Lu, X Yan, Y Wang, J Ma… - Journal of Agricultural …, 2021 - ACS Publications
Shading was thought as an effective approach to increase theanine in harvested tea shoots.
Previous studies offered conflicting findings, perhaps since the integration of theanine …

Glutamine synthetases play a vital role in high accumulation of theanine in tender shoots of albino tea germplasm “Huabai 1”

Y Yu, X Kou, R Gao, X Chen, Z Zhao… - Journal of agricultural …, 2021 - ACS Publications
Theanine (N-ethyl-γ-l-glutamine) is a special nonprotein amino acid that contributes to the
umami taste and health function of tea. Although recent studies on tea breeding have …

Strategies for studying in vivo biochemical formation pathways and multilevel distributions of quality or function-related specialized metabolites in tea (Camellia …

Y Liao, X Fu, L Zeng, Z Yang - Critical Reviews in Food Science …, 2021 - Taylor & Francis
Tea (Camellia sinensis) contains bioactive metabolites such as catechins, amino acids,
caffeine, and aroma compounds that contribute to characteristic tea function and flavor …

Effects of long-term nitrogen fertilization on the formation of metabolites related to tea quality in subtropical China

Y Chen, F Wang, Z Wu, F Jiang, W Yu, J Yang, J Chen… - Metabolites, 2021 - mdpi.com
As a main agronomic intervention in tea cultivation, nitrogen (N) application is useful to
improve tea yield and quality. However, the effects of N application on the formation of tea …

Theanine Improves Salt Stress Tolerance via Modulating Redox Homeostasis in Tea Plants (Camellia sinensis L.)

Z Chen, S Lin, J Li, T Chen, Q Gu, T Yang… - Frontiers in Plant …, 2021 - frontiersin.org
Theanine, a unique non-proteinogenic amino acid, is one of the most abundant secondary
metabolites in tea. Its content largely determines green tea quality and price. However, its …

Production of l-Theanine by Escherichia coli in the Absence of Supplemental Ethylamine

R Hagihara, S Ohno, M Hayashi… - Applied and …, 2021 - Am Soc Microbiol
ABSTRACT l-Theanine is a nonproteinogenic amino acid present almost exclusively in tea
plants and is beneficial for human health. For industrial production, l-theanine is …

[HTML][HTML] Isolation of mesophyll protoplasts from tea (Camellia sinensis) and localization analysis of enzymes involved in the biosynthesis of specialized metabolites

Y Zhou, R Deng, X Xu, Z Yang - Beverage Plant Research, 2021 - maxapress.com
Specialized metabolites in tea (Camellia sinensis) are fundamental quality factors. It is
important to characterize gene function in vivo to identify key enzymes and reactions …

Stable Isotope-Labeled Precursor Tracing Reveals that l-Alanine is Converted to l-Theanine via l-Glutamate not Ethylamine in Tea Plants In Vivo

X Fu, Y Liao, S Cheng, R Deng… - Journal of Agricultural and …, 2021 - ACS Publications
Tea plants (Camellia sinensis) specifically produce l-theanine, which contributes to tea
function and taste. Ethylamine is a limiting factor differentiating l-theanine accumulation …

Tonoplast-Localized Theanine Transporter CsCAT2 May Mediate Theanine Storage in the Root of Tea Plants (Camellia sinensis L.)

L Feng, Y Yu, S Lin, T Yang, Q Chen, L Liu… - Frontiers in Plant …, 2021 - frontiersin.org
Theanine is the component endowing tea infusion with “umami” taste and antidepression
benefits. Theanine is primarily synthesized and stored in root in winter and is transported via …