Ancient microbes invented biochemical mechanisms and assembled core metabolic
pathways on an anoxic Earth. Molecular oxygen appeared far later, forcing microbes to …

Since the 20th century, plastics that are widely being used in general life and industries are
causing enormous plastic waste problems since improperly discarded plastics barely …

In [NiFe]-hydrogenases, the active-site Ni is coordinated by four cysteine-S ligands (Cys; C),
two of which are bridging to the Fe (CO)(CN) 2 fragment. Substitution of a single Cys residue …

Controlled formation of catalytically-relevant states within crystals of complex
metalloenzymes represents a significant challenge to structure–function studies. Here we …

Abstract Iron–sulfur (Fe–S) centers are versatile building blocks in biological electron
transfer chains because their redox potentials may cover a wide potential range depending …

Nature provides us with the most suitable and efficient biocatalysts in the form of redox
enzymes to perform a variety of catalytic reactions [1]. While some enzymes are capable of …

Hydrogenazy to multimeryczne metaloenzymy, katalizujące odwracalną reakcję redukcji
protonów do wodoru cząsteczkowego. Obecnie hydrogenazy próbuje się wykorzystywać …

[FeFe]-hydrogenase is nature's most efficient proton reducing and H2 oxidising enzyme.
However, biotechnological applications of the enzyme are currently restricted due to its …

Hydrogenazy to multimeryczne metaloenzymy, katalizujące odwracalną reakcję redukcji
protonów do wodoru cząsteczkowego. Obecnie hydrogenazy próbuje się wykorzystywać …

In this work we report the first in vitro reconstitution of the complex catalytic center of a [NiFe]-
hydrogenase. The strategy is based on the well-known membrane-bound [NiFe] …