Mosquito-borne diseases, such as dengue and malaria, pose significant global health
burdens. Unfortunately, current control methods based on insecticides and environmental …

The CRISPR-Cas system is a powerful tool for in vivo editing the genome of most organisms,
including man. During the years this technique has been applied in several fields, such as …

The mosquito Aedes aegypti is the principal vector for arboviruses including dengue/yellow
fever, chikungunya, and Zika virus, infecting hundreds of millions of people annually …

Synthetic gene drive constructs could, in principle, provide the basis for highly efficient
interventions to control disease vectors and other pest species. This efficiency derives in part …

Insects play important roles as predators, prey, pollinators, recyclers, hosts, parasitoids, and
sources of economically important products. They can also destroy crops; wound animals; …

Gene drive elements promote the spread of linked traits, providing methods for changing the
composition or fate of wild populations. Drive mechanisms that are self-limiting are attractive …

Engineered reproductive species barriers are useful for impeding gene flow and driving
desirable genes into wild populations in a reversible threshold-dependent manner …

CRISPR-Cas9 mediated genome editing methods are being used for the analysis of gene
function. However, it is hard to identify gene knockout mutants for genes whose knockout …

The discovery of CRISPR-based gene editing and its application to homing-based gene
drive has been greeted with excitement, for its potential to control mosquito-borne diseases …

Genome-editing methods are becoming routine tools for molecular and cell biologists. Such
tools include ZFNs, CRISPR, megaTALs and TALENs. These tools are revolutionizing the …