Understanding tumor immune microenvironments is critical for identifying immune modifiers
of cancer progression and developing cancer immunotherapies. Recent applications of …

Single-cell approaches are quickly changing our view on biological systems by increasing
the spatiotemporal resolution of our analyses to the level of the individual cell. The field of …

Fibrotic skin disease represents a major global healthcare burden, characterized by
fibroblast hyperproliferation and excessive accumulation of extracellular matrix. Fibroblasts …

Microglia, the resident immune cells of the brain, have emerged as crucial regulators of
synaptic refinement and brain wiring. However, whether the remodeling of distinct synapse …

High-throughput amplicon sequencing of large genomic regions remains challenging for
short-read technologies. Here, we report a high-throughput amplicon sequencing approach …

Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Single-cell RNA sequencing (scRNA-seq) is generally used for profiling transcriptome of
individual cells. The droplet-based 10X Genomics Chromium (10X) approach and the plate …

Single-cell technologies are emerging as powerful tools for cancer research. These
technologies characterize the molecular state of each cell within a tumor, enabling new …

The high proportion of zeros in typical single-cell RNA sequencing datasets has led to
widespread but inconsistent use of terminology such as dropout and missing data. Here, we …

Rapid cost drops and advancements in next-generation sequencing have made profiling of
cells at individual level a conventional practice in scientific laboratories worldwide. Single …