CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …

Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …

In the last years, tremendous progress has been made in the development of CRISPR/Cas-
mediated genome editing tools. A number of natural CRISPR/Cas nuclease variants have …

High‐oleic acid content, nontransgenic allotetraploid cotton (Gossypium hirsutum L.) generated
by knockout of GhFAD2 genes with CRISPR/Cas9 system - PMC Back to Top Skip to main …

The creation of new soybean varieties has been limited by genomic duplication and
redundancy. Efficient multiplex gene editing and large chromosomal segment deletion …

Weeds have continually interrupted crop plants since their domestication, leading to a
greater yield loss compared to diseases and pests that necessitated the practice of weed …

Capturing heterosis (hybrid vigor) is a promising way to increase productivity in many crops;
hybrid crops often have superior yields, disease resistance, and stress tolerance compared …

As a tool for modifying the genome, gene editing technology has developed rapidly in recent
years, especially in the past two years. With the emergence of new gene editing …

Oil crops, mainly comprised of soybean, rapeseed, groundnut, sunflower and etc., have
provided substantial edible oil and other tremendous nutrients for human beings, as well as …