The phasor approach to fluorescence lifetime imaging, and more recently hyperspectral
fluorescence imaging, has increased the use of these techniques, and improved the ease …

Adipocytes are the main cell type in adipose tissue, which is a critical regulator of
metabolism, highly specialized in storing energy as fat. Adipocytes differentiate from …

Macrophages (ΜΦs) are important immune effector cells that promote (M1 ΜΦs) or inhibit
(M2 ΜΦs) inflammation and are involved in numerous physiological and pathogenic …

Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast
mechanism to monitor biological parameters and molecular events in vivo. Single-photon …

The excited-state lifetime is an intrinsic property of fluorescent molecules that can be
leveraged for multiplexed imaging. An advantage of fluorescence lifetime-based …

Multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) is extensively proposed
as a non-invasive optical method to study tissue metabolism. The approach is based on …

Type 1 Natural Killer T-cells (NKT1 cells) play a critical role in mediating hepatic ischemia-
reperfusion injury (IRI). Although hepatic steatosis is a major risk factor for preservation type …

Innate lymphoid cells (ILCs), the most recently described family of lymphoid cells, play
fundamental roles in tissue homeostasis through the production of key cytokine. Group 1 …

Corals and their photosynthetic endosymbiotic algae (Symbiodiniaceae) produce a strong
autofluorescent signal that spans the visible to near-infrared (NIR) spectrum. However, this …

We investigated the possibility of using long excitation pulses in fluorescence lifetime
imaging microscopy (FLIM) using phasor analysis. It has long been believed that the pulse …