Statistics or biology: the zero-inflation controversy about scRNA-seq data

R Jiang, T Sun, D Song, JJ Li - Genome biology, 2022 - Springer
Researchers view vast zeros in single-cell RNA-seq data differently: some regard zeros as
biological signals representing no or low gene expression, while others regard zeros as …

Recent developments in application of single-cell RNA sequencing in the tumour immune microenvironment and cancer therapy

PH Li, XY Kong, YZ He, Y Liu, X Peng, ZH Li… - Military Medical …, 2022 - Springer
The advent of single-cell RNA sequencing (scRNA-seq) has provided insight into the tumour
immune microenvironment (TIME). This review focuses on the application of scRNA-seq in …

Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation

AD Brunner, M Thielert, C Vasilopoulou… - Molecular systems …, 2022 - embopress.org
Single‐cell technologies are revolutionizing biology but are today mainly limited to imaging
and deep sequencing. However, proteins are the main drivers of cellular function and in …

Comparison and evaluation of statistical error models for scRNA-seq

S Choudhary, R Satija - Genome biology, 2022 - Springer
Background Heterogeneity in single-cell RNA-seq (scRNA-seq) data is driven by multiple
sources, including biological variation in cellular state as well as technical variation …

Guidelines for bioinformatics of single-cell sequencing data analysis in Alzheimer's disease: review, recommendation, implementation and application

M Wang, W Song, C Ming, Q Wang, X Zhou… - Molecular …, 2022 - Springer
Alzheimer's disease (AD) is the most common form of dementia, characterized by
progressive cognitive impairment and neurodegeneration. Extensive clinical and genomic …

Single-cell RNA sequencing with spatial transcriptomics of cancer tissues

R Ahmed, T Zaman, F Chowdhury, F Mraiche… - International journal of …, 2022 - mdpi.com
Single-cell RNA sequencing (RNA-seq) techniques can perform analysis of transcriptome at
the single-cell level and possess an unprecedented potential for exploring signatures …

[HTML][HTML] Upcoming progress of transcriptomics studies on plants: An overview

P Tyagi, D Singh, S Mathur, A Singh… - Frontiers in Plant …, 2022 - frontiersin.org
Transcriptome sequencing or RNA-Sequencing is a high-resolution, sensitive and high-
throughput next-generation sequencing (NGS) approach used to study non-model plants …

[HTML][HTML] Unravelling glioblastoma heterogeneity by means of single-cell RNA sequencing

AH Martínez, R Madurga, N García-Romero… - Cancer letters, 2022 - Elsevier
Glioblastoma (GBM) is the most invasive and deadliest brain cancer in adults. Its inherent
heterogeneity has been designated as the main cause of treatment failure. Thus, a deeper …

Heterogeneity in endothelial cells and widespread venous arterialization during early vascular development in mammals

S Hou, Z Li, J Dong, Y Gao, Z Chang, X Ding, S Li, Y Li… - Cell research, 2022 - nature.com
Arteriogenesis rather than unspecialized capillary expansion is critical for restoring effective
circulation to compromised tissues in patients. Deciphering the origin and specification of …

Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models

K Song, X Yang, G An, X Xia, J Zhao, X Xu… - Nature …, 2022 - nature.com
Type 2 diabetes mellitus is one of the most prevalent metabolic diseases presenting with
systemic pathologies, including reproductive disorders in male diabetic patients. However …