Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox
CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …
effector mechanisms and have been repurposed for versatile applications in therapeutics …
Gold nanomaterials‐implemented CRISPR‐Cas systems for biosensing
R Fu, Y Xianyu - Small, 2023 - Wiley Online Library
Due to their superiority in the simple design and precise targeting, clustered regularly
interspaced short palindromic repeats (CRISPR)‐Cas systems have attracted significant …
interspaced short palindromic repeats (CRISPR)‐Cas systems have attracted significant …
TnpB structure reveals minimal functional core of Cas12 nuclease family
G Sasnauskas, G Tamulaitiene, G Druteika, A Carabias… - Nature, 2023 - nature.com
The widespread TnpB proteins of IS200/IS605 transposon family have recently emerged as
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …
the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells …
An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity
Compact CRISPR-Cas systems offer versatile treatment options for genetic disorders, but
their application is often limited by modest gene-editing activity. Here we present …
their application is often limited by modest gene-editing activity. Here we present …
Cas12n nucleases, early evolutionary intermediates of type V CRISPR, comprise a distinct family of miniature genome editors
W Chen, J Ma, Z Wu, Z Wang, H Zhang, W Fu, D Pan… - Molecular cell, 2023 - cell.com
Type V CRISPR-associated systems (Cas) 12 family nucleases are considered to have
evolved from transposon-associated TnpB, and several of these nucleases have been …
evolved from transposon-associated TnpB, and several of these nucleases have been …
CRISPR–Cas12‐based field‐deployable system for rapid detection of synthetic DNA sequence of the monkeypox virus genome
The global outbreak of the monkeypox virus (MPXV) highlights the need for rapid and cost‐
effective MPXV detection tools to effectively monitor and control the monkeypox disease …
effective MPXV detection tools to effectively monitor and control the monkeypox disease …
Drug delivery systems for CRISPR-based genome editors
V Madigan, F Zhang, JE Dahlman - Nature Reviews Drug Discovery, 2023 - nature.com
CRISPR-based drugs can theoretically manipulate any genetic target. In practice, however,
these drugs must enter the desired cell without eliciting an unwanted immune response, so …
these drugs must enter the desired cell without eliciting an unwanted immune response, so …
[HTML][HTML] Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges
Clustered regulatory interspaced short palindromic repeats (CRISPR) has changed
biomedical research and provided entirely new models to analyze every aspect of …
biomedical research and provided entirely new models to analyze every aspect of …
The expanded CRISPR toolbox for constructing microbial cell factories
Microbial cell factories (MCFs) convert low-cost carbon sources into valuable compounds.
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …
CRISPRimmunity: an interactive web server for CRISPR-associated Important Molecular events and Modulators Used in geNome edIting Tool identifYing
F Zhou, X Yu, R Gan, K Ren, C Chen… - Nucleic acids …, 2023 - academic.oup.com
The CRISPR-Cas system is a highly adaptive and RNA-guided immune system found in
bacteria and archaea, which has applications as a genome editing tool and is a valuable …
bacteria and archaea, which has applications as a genome editing tool and is a valuable …