Navigation through the bulk tumour, entry into the blood vasculature, survival in the
circulation, exit at distant sites and resumption of proliferation are all steps necessary for …

Optogenetics is widely used to interrogate the neural circuits underlying disease and has
most recently been harnessed for therapeutic applications. The optogenetic toolkit consists …

The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast
timescale. The structural dynamics that result from femtosecond optical excitation have …

Aberrant kinase activity contributes to the pathogenesis of brain cancers,
neurodegeneration, and neuropsychiatric diseases, but identifying kinase inhibitors that …

The innovative advances in transforming clustered regularly interspaced short palindromic
repeats-associated protein 9 (CRISPR/Cas9) into different variants have taken the art of …

Background Opsin‐based optogenetics has emerged as a powerful biomedical tool using
light to control protein conformation. Such capacity has been initially demonstrated to control …

Precise regulation of protein activity and localization in cancer cells is crucial to dissect the
function of the protein‐involved cellular network in tumorigenesis, but there is a lack of …

In this article, we provide detailed protocols on using optogenetic dimerizers to acutely
perturb activities of guanine nucleotide exchange factors (GEFs) specific to Ras, Rac or Rho …

The photoacid dynamics of fluorescent proteins include both electronic excited-and ground-
state mechanisms of proton transfer. The associated characteristic timescales of these …

The dysregulation of protein kinases is associated with multiple diseases due to the kinases'
involvement in a variety of cell signaling pathways. Manipulating protein kinase function, by …