CryoEM reveals the structure of an archaeal pilus involved in twitching motility

MC Gaines, S Sivabalasarma, MN Isupov… - Nature …, 2024 - nature.com
Amongst the major types of archaeal filaments, several have been shown to closely
resemble bacterial homologues of the Type IV pili (T4P). Within Sulfolobales, member …

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y

L Kavčič, A Kežar, N Koritnik, MT Žnidarič… - Communications …, 2024 - nature.com
The structural diversity and tunability of the capsid proteins (CPs) of various icosahedral and
rod-shaped viruses have been well studied and exploited in the development of smart …

Flagellar point mutation causes social aggregation in laboratory-adapted Bacillus subtilis under conditions that promote swimming

S Alvi, VD Mondelo, J Boyle, A Buck, J Gejo… - Journal of …, 2024 - journals.asm.org
Motility allows microbes to explore and maximize success in their environment; however,
many laboratory bacterial strains have a reduced or altered capacity for motility. Swimming …

Response of Worm-like Chains to Traveling-Wave Active Forces

F Cecconi, D Lucente, A Puglisi, M Viale - arXiv preprint arXiv:2410.00864, 2024 - arxiv.org
We present a simplified theory for semiflexible flagella under the action of a traveling-wave
perturbation that emulates the organized active forces generated by molecular motors …

Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus

H Ochner, J Boehning, Z Wang, AK Tarafder, I Caspy… - bioRxiv, 2024 - biorxiv.org
Type IV pili (T4Ps), which are abundant in many bacterial and archaeal species, have been
shown to play important roles in both surface sensing and twitching motility, with implications …

[HTML][HTML] Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages

JC Sanchez, JK Baumgardt, ER Wright - Bio-protocol, 2024 - ncbi.nlm.nih.gov
A number of extracellular helical protein polymers are crucial for supporting bacterial
motility. The bacterial flagellum is a polymeric appendage used to support cellular motility …

[PDF][PDF] Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines

A Deniaud, BV Kabasakal, JC Bufton… - … for Protein Complex …, 2024 - library.oapen.org
High-resolution structure determination by electron cryo-microscopy underwent a step
change in recent years. This now allows study of challenging samples which previously …

Elucidation of the glycan structure of the b-type flagellin of Pseudomonas aeruginosa PAO1

PJ Hensbergen, L van Huijkelom, J van Angeren… - bioRxiv, 2024 - biorxiv.org
Flagella are essential for motility and pathogenicity in many bacteria. The main component
of the flagellar filament, flagellin, often undergoes post-translational modifications, with …