Phthiocerol dimycocerosate (PDIM) is an essential virulence lipid of Mycobacterium
tuberculosis. In vitro culturing rapidly selects for spontaneous PDIM-negative mutants that …

Human and animal tuberculosis is caused by the Mycobacterium tuberculosis Complex
(MTBC), which has evolved a genomic decay of cobalamin (vitamin B12) biosynthetic …

ABSTRACT Vitamin B12 (B12) serves as a critical cofactor within mycobacterial metabolism.
While some pathogenic strains can synthesize B12 de novo, others rely on host-acquired …

BacA is a mycobacterial ATP-binding cassette (ABC) transporter involved in the
translocation of water-soluble compounds across the lipid bilayer. Whole-cell-based assays …

Vitamin B12 is an essential cofactor in all domains of life and B12-sensing riboswitches are
some of the most widely distributed riboswitches. Mycobacterium tuberculosis, the causative …

BtuM is a bacterial cobalamin transporter that binds the transported substrate in the base-off
state, with a cysteine residue providing the α-axial coordination of the central cobalt ion via a …

Antibodies are important tools for the study of membrane proteins in biological, biophysics
and structural analyses. However, the production of purified membrane proteins is still a …

In this thesis we mainly focus on membrane transporters that facilitate the transport of
vitamin B12 in bacteria. One of these transporters is the ABC transporters BacA from …