Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …

Simple, efficient and well-tolerated delivery of CRISPR genome editing systems into primary
cells remains a major challenge. Here we describe an engineered Peptide-Assisted …

Mutations in the BRCA2 gene are associated with sporadic and familial cancer, cause
genomic instability and sensitize cancer cells to inhibition by the poly (ADP-ribose) …

CRISPR–Cas9 genome engineering is a powerful technology for correcting genetic
diseases. However, the targeting range of Cas9 proteins is limited by their requirement for a …

Genetic interactions mediate the emergence of phenotype from genotype, but technologies
for combinatorial genetic perturbation in mammalian cells are challenging to scale. Here, we …

Genome editing with prime editors based on CRISPR-Cas9 is limited by the large size of the
system and the requirement for a G/C-rich protospacer-adjacent motif (PAM) sequence …

CRISPR–Cas12f nucleases are currently one of the smallest genome editors, exhibiting
advantages for efficient delivery via cargo-size-limited adeno-associated virus delivery …

Adenine base editors (ABEs) are precise gene-editing agents that convert A: T pairs into G:
C through a deoxyinosine intermediate. Existing ABEs function most effectively when the …

Functional studies of long noncoding RNAs (lncRNAs) have been hindered by the lack of
methods to assess their evolution. Here we present lncRNA Homology Explorer (lncHOME) …