Bacterial genome engineering using CRISPR-associated transposases
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated
transposases have the potential to transform the technology landscape for kilobase-scale …
transposases have the potential to transform the technology landscape for kilobase-scale …
CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications
In the past decades, the broad selection of CRISPR-Cas systems has revolutionized
biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in …
biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in …
An efficient in vivo-inducible CRISPR interference system for group A Streptococcus genetic analysis and pathogenesis studies
While genome-wide transposon mutagenesis screens have identified numerous essential
genes in the significant human pathogen Streptococcus pyogenes (group A Streptococcus …
genes in the significant human pathogen Streptococcus pyogenes (group A Streptococcus …
CRISPR-Cas tools for simultaneous transcription & translation control in bacteria
RAL Cardiff, ID Faulkner, JG Beall… - Nucleic Acids …, 2024 - academic.oup.com
Robust control over gene translation at arbitrary mRNA targets is an outstanding challenge
in microbial synthetic biology. The development of tools that can regulate translation will …
in microbial synthetic biology. The development of tools that can regulate translation will …
[HTML][HTML] Current approaches to genetic modification of marine bacteria and considerations for improved transformation efficiency
Marine bacteria play vital roles in symbiosis, biogeochemical cycles and produce novel
bioactive compounds and enzymes of interest for the pharmaceutical, biofuel and …
bioactive compounds and enzymes of interest for the pharmaceutical, biofuel and …