Deconstructing synthetic biology across scales: a conceptual approach for training synthetic biologists

AS Karim, DM Brown, CM Archuleta, S Grannan… - Nature …, 2024 - nature.com
Synthetic biology allows us to reuse, repurpose, and reconfigure biological systems to
address society's most pressing challenges. Developing biotechnologies in this way …

One-Pot Sequential Two-Step Photo-Biocatalytic Deracemization of sec-Alcohols Combining Photocatalytic Oxidation and Bioreduction

A Rudzka, N Antos, T Reiter, W Kroutil… - ACS …, 2024 - ACS Publications
Chiral alcohols are versatile building blocks and are of particular interest in the asymmetric
synthesis of nonracemic active pharmaceutical ingredients, agrochemicals, fragrances …

Antibacterial Action of Zn2+ Ions Driven by the In Vivo Formed ZnO Nanoparticles

T Vitasovic, G Caniglia, N Eghtesadi… - … Applied Materials & …, 2024 - ACS Publications
Antibacterial formulations based on zinc oxide nanoparticles (ZnO NPs) are widely used for
antibiotic replacement in veterinary medicine and animal nutrition. However, the undesired …

Deciphering pathogens inactivation mechanism during anaerobic co-digestion of food waste and waste activated sludge: The role of pH

R Tang, Q Zhang, YD Chen, WJ Bai, JJ Xu… - Chemical Engineering …, 2024 - Elsevier
Anaerobic co-digestion of waste activated sludge (WAS) with food waste (FW) represents a
viable and effective approach to energy recovery and pollutant elimination. Little is known …

Metabolic disruption impairs ribosomal protein levels, resulting in enhanced aminoglycoside tolerance

R Shiraliyev, MA Orman - Elife, 2024 - elifesciences.org
Aminoglycoside antibiotics target ribosomes and are effective against a wide range of
bacteria. Here, we demonstrated that knockout strains related to energy metabolism in …

Active pH regulation facilitates Bacillus subtilis biofilm development in a minimally buffered environment

P Tran, SM Lander, A Prindle - Mbio, 2024 - Am Soc Microbiol
Biofilms provide individual bacteria with many advantages, yet dense cellular proliferation
can also create intrinsic metabolic challenges including excessive acidification. Because …

A riboswitch-controlled TerC family transporter Alx tunes intracellular manganese concentration in Escherichia coli at alkaline pH

R Sharma, TV Mishanina - Journal of Bacteriology, 2024 - Am Soc Microbiol
Cells use transition metal ions as structural components of biomolecules and cofactors in
enzymatic reactions, making transition metal ions integral cellular components. Organisms …

Fitness trade-offs of multidrug efflux pumps in Escherichia coli K-12 in acid or base, and with aromatic phytochemicals

Y Liu, AM Van Horn, MTN Pham… - Applied and …, 2024 - Am Soc Microbiol
Multidrug efflux pumps are the frontline defense mechanisms of Gram-negative bacteria, yet
little is known of their relative fitness trade-offs under gut conditions such as low pH and the …

Improved self-cleaving precipitation tags for efficient column free bioseparations

H Yuan, SV Prabhala, MJ Coolbaugh… - Protein Expression and …, 2024 - Elsevier
Current biological research requires simple protein bioseparation methods capable of
purifying target proteins in a single step with high yields and purities. Conventional affinity …

Towards optical measurements of membrane potential values in Bacillus subtilis using fluorescence lifetime

D Roy, X Michalet, EW Miller, S Weiss - bioRxiv, 2024 - biorxiv.org
Membrane potential (MP) changes can provide a simple readout of bacterial functional and
metabolic state or stress levels. While several optical methods exist for measuring fast …