The eukaryotic cell cycle comprises a series of events, whose ordering and correct
progression depends on the oscillating activity of cyclin-dependent kinases (Cdks), which …

DNA replication forks pause in front of lesions on the template, eventually leading to
cytotoxic chromosomal rearrangements. The in vivo structure of damaged eukaryotic …

The heterotrimeric checkpoint clamp comprises the Saccharomyces cerevisiae Rad17,
Mec3, and Ddc1 subunits (Rad17/3/1, the 9-1-1 complex in humans). This DNA damage …

When eukaryotic chromosomes undergo double strand breaks (DSBs), several
evolutionarily conserved proteins, among which the MRX complex, are recruited to the break …

Previous work on the DNA damage checkpoint in Saccharomyces cerevisiae has shown that
two complexes independently sense DNA lesions: the kinase Mec1-Ddc2 and the PCNA …

Damage tolerance mechanisms mediating damage-bypass and gap-filling are crucial for
genome integrity. A major damage tolerance pathway involves recombination and is …

Rad51, Rad52, and RPA play central roles in homologous DNA recombination. Rad51
mediates DNA strand exchange, a key reaction in DNA recombination. Rad52 has two …

To protect the genome, cells have evolved a diverse set of pathways designed to sense,
signal, and repair multiple types of DNA damage. To assess the degree of coordination and …

DNA double‐strand breaks (DSBs) are among the most deleterious types of damage that
can occur in the genome of eukaryotic cells because failure to repair them can lead to loss of …

The DNA damage checkpoint and the spindle assembly checkpoint (SAC) are two important
regulatory mechanisms that respond to different lesions. The DNA damage checkpoint …