Nuclear cloning and epigenetic reprogramming of the genome

WM Rideout III, K Eggan, R Jaenisch - Science, 2001 - science.org
Cloning of mammals by nuclear transfer (NT) results in gestational or neonatal failure with at
most a few percent of manipulated embryos resulting in live births. Many of those that …

Bovine embryo technologies

C Galli, R Duchi, G Crotti, P Turini, N Ponderato… - Theriogenology, 2003 - Elsevier
Embryo technologies are a combination of assisted reproduction, cellular and molecular
biology and genomic techniques. Their classical use in animal breeding has been to …

Frequency and occurrence of late-gestation losses from cattle cloned embryos

Y Heyman, P Chavatte-Palmer… - Biology of …, 2002 - academic.oup.com
Nuclear transfer from somatic cells still has limited efficiency in terms of live calves born due
to high fetal loss after transfer. In this study, we addressed the type of donor cells used for …

[HTML][HTML] A sheep model of cystic fibrosis generated by CRISPR/Cas9 disruption of the CFTR gene

Z Fan, IV Perisse, CU Cotton, M Regouski, Q Meng… - JCI insight, 2018 - ncbi.nlm.nih.gov
Cystic fibrosis (CF) is a genetic disease caused by mutations in the CF transmembrane
conductance regulator (CFTR) gene. The major cause of limited life span in CF patients is …

Epigenetic characteristics and development of embryos cloned from donor cells treated by trichostatin A or 5-aza-2′-deoxycytidine

BP Enright, C Kubota, X Yang, XC Tian - Biology of reproduction, 2003 - academic.oup.com
Abstract Development to blastocyst following nuclear transfer is dependent on the donor
cell's ability to reprogram its genome to that of a zygote. This reprogramming step is …

Lessons learned from somatic cell nuclear transfer

C Gouveia, C Huyser, D Egli, MS Pepper - International journal of …, 2020 - mdpi.com
Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell
research and regenerative medicine for the past 20 years. The main biological goal of SCNT …

The health profile of cloned animals

JB Cibelli, KH Campbell, GE Seidel, MD West… - Nature …, 2002 - nature.com
14 develop abnormally is currently unjustified on scientific grounds and should not be used
by policymakers or other groups as an argument to ban all human cloning research. In our …

Producing proteins in transgenic plants and animals

JW Larrick, DW Thomas - Current opinion in biotechnology, 2001 - Elsevier
The requirement for large quantities of therapeutic proteins has fuelled interest in the
production of recombinant proteins in plants and animals. The first commercial products to …

Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes

XJ Yin, T Tani, I Yonemura, M Kawakami… - Biology of …, 2002 - academic.oup.com
The present study demonstrated that brief treatment of in vitro-matured porcine oocytes with
demecolcine results in a membrane protrusion that contains a condensed chromosome …

Production of cloned pigs by using somatic cells as donors

L Lai, RS Prather - Cloning & Stem Cells, 2003 - liebertpub.com
NUCLEAR TRANSFER is a procedure by which genetically identical individuals can be
created. The applications of these techniques for nuclear transfer will be in agricultural …