Cell electrofusion in microfluidic devices attracted great attention in recent years due to its
widespread applications potential in cell-based studies. In these microfluidic devices, many …

Embryologists working with livestock species were the pioneers in the field of
reprogramming by somatic cell nuclear transfer (SCNT). Without the “Dolly experiment,” the …

Protocols for the successful manipulation and preservation of semen in a given species
depend upon a fundamental knowledge of how spermatozoa respond to the …

Taking into account the latest Red List of the International Union for Conservation of Nature
in which 25% of all mammals are threatened with extinction, somatic cell nuclear transfer …

Nuclear transfer (NT) remains the most effective method to reprogram somatic cells to
totipotency. Somatic cell nuclear transfer (SCNT) efficiency however remains low, but …

More than a decade has now passed since the birth of the first endangered species
produced from an adult somatic cell reprogrammed by somatic cell nuclear transfer. At that …

In studies carried out previously, we demonstrated that small ubiquitin-like modifier 1
(SUMO1) is associated with poor sperm motility when evaluated with a protocol that reveals …

The success of cloned animal “Dolly Sheep” demonstrated the somatic cell nuclear transfer
(SCNT) technique holds huge potentials for mammalian asexual reproduction. However, the …

Skin fibroblast cells were obtained from a small piece of an ear of leopard, lion, and tiger
collected postmortem and attempts were made to synchronize the skin fibroblasts at G0/G1 …

Incompatibilities between the nucleus and the cytoplasm of sufficiently distant species result
in developmental arrest of hybrid and nucleocytoplasmic hybrid (cybrid) embryos. Several …