Glutaraldehyde in bio-catalysts design: A useful crosslinker and a versatile tool in enzyme immobilization

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Rsc Advances, 2014 - pubs.rsc.org
Glutaraldehyde is one of the most widely used reagents in the design of biocatalysts. It is a
powerful crosslinker, able to react with itself, with the advantages that this may bring forth. In …

[HTML][HTML] Environmental assessment of enzyme use in industrial production–a literature review

KR Jegannathan, PH Nielsen - Journal of cleaner production, 2013 - Elsevier
Enzymatic processes have been implemented in a broad range of industries in recent
decades because they are specific, fast in action and often save raw materials, energy …

Strategies for stabilization of enzymes in organic solvents

V Stepankova, S Bidmanova, T Koudelakova… - Acs …, 2013 - ACS Publications
One of the major barriers to the use of enzymes in industrial biotechnology is their
insufficient stability under processing conditions. The use of organic solvent systems instead …

Directed evolution drives the next generation of biocatalysts

NJ Turner - Nature chemical biology, 2009 - nature.com
Enzymes are increasingly being used as biocatalysts in the generation of products that have
until now been derived using traditional chemical processes. Such products range from …

New generation of biocatalysts for organic synthesis

BM Nestl, SC Hammer, BA Nebel… - Angewandte Chemie …, 2014 - Wiley Online Library
The use of enzymes as catalysts for the preparation of novel compounds has received
steadily increasing attention over the past few years. High demands are placed on the …

Directed evolution of enzymes for industrial biocatalysis

JL Porter, RA Rusli, DL Ollis - ChemBioChem, 2016 - Wiley Online Library
Enzymes have the potential to catalyse a wide variety of chemical reactions. They are
increasingly being sought as environmentally friendly and cost‐effective alternatives to …

Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges

JD Cui, SR Jia - Critical reviews in biotechnology, 2015 - Taylor & Francis
Cross-linked enzyme aggregate (CLEA) technology has been regarded as an effective
carrier-free immobilization method. This method is very attractive due to its simplicity and …

High-yield hydrogen production from biomass by in vitro metabolic engineering: mixed sugars coutilization and kinetic modeling

JA Rollin, J Martin del Campo… - Proceedings of the …, 2015 - National Acad Sciences
The use of hydrogen (H2) as a fuel offers enhanced energy conversion efficiency and
tremendous potential to decrease greenhouse gas emissions, but producing it in a …

Directed evolution of enzyme stability

VGH Eijsink, S Gåseidnes, TV Borchert… - Biomolecular …, 2005 - Elsevier
Modern enzyme development relies to an increasing extent on strategies based on diversity
generation followed by screening for variants with optimised properties. In principle, these …

Biocatalytic reductions and chemical versatility of the old yellow enzyme family of flavoprotein oxidoreductases

HS Toogood, JM Gardiner, NS Scrutton - ChemCatChem, 2010 - Wiley Online Library
The old yellow enzyme (OYE) family is a large group of flavin‐dependent redox biocatalysts
with major applications in the industrial reduction of activated alkenes. These enzymes have …