Nonlinear optical microscopy: From fundamentals to applications in live bioimaging
A recent challenge in the field of bioimaging is to image vital, thick, and complex tissues in
real time and in non-invasive mode. Among the different tools available for diagnostics …
real time and in non-invasive mode. Among the different tools available for diagnostics …
Broadband coherent Raman scattering microscopy
Spontaneous Raman (SR) microscopy allows label‐free chemically specific imaging based
on the vibrational response of molecules; however, due to the low Raman scattering cross …
on the vibrational response of molecules; however, due to the low Raman scattering cross …
Weakly supervised identification and localization of drug fingerprints based on label-free hyperspectral CARS microscopy
Understanding drug fingerprints in complex biological samples is essential for the
development of a drug. Hyperspectral coherent anti-Stokes Raman scattering (HS-CARS) …
development of a drug. Hyperspectral coherent anti-Stokes Raman scattering (HS-CARS) …
Perspective: Coherent Raman scattering microscopy, the future is bright
Chemical imaging offers critical information to understand the fundamentals in biology and
to assist clinical diagnostics. Label-free chemical imaging piques a general interest since it …
to assist clinical diagnostics. Label-free chemical imaging piques a general interest since it …
Quantitative chemical imaging and unsupervised analysis using hyperspectral coherent anti-Stokes Raman scattering microscopy
In this work, we report a method to acquire and analyze hyperspectral coherent anti-Stokes
Raman scattering (CARS) microscopy images of organic materials and biological samples …
Raman scattering (CARS) microscopy images of organic materials and biological samples …
Nonlinear vibrational microscopy applied to lipid biology
A Zumbusch, W Langbein, P Borri - Progress in Lipid Research, 2013 - Elsevier
Optical microscopy is an indispensable tool that is driving progress in cell biology. It still is
the only practical means of obtaining spatial and temporal resolution within living cells and …
the only practical means of obtaining spatial and temporal resolution within living cells and …
Quantitative imaging of lipids in live mouse oocytes and early embryos using CARS microscopy
J Bradley, I Pope, F Masia, R Sanusi… - …, 2016 - journals.biologists.com
Mammalian oocytes contain lipid droplets that are a store of fatty acids, whose metabolism
plays a substantial role in pre-implantation development. Fluorescent staining has …
plays a substantial role in pre-implantation development. Fluorescent staining has …
Coherent anti-Stokes Raman scattering microscopy of single nanodiamonds
Nanoparticles have attracted enormous attention for biomedical applications as optical
labels, drug-delivery vehicles and contrast agents in vivo. In the quest for superior …
labels, drug-delivery vehicles and contrast agents in vivo. In the quest for superior …
Dissecting lipid droplet biology with coherent Raman scattering microscopy
Lipid droplets (LDs) are lipid-rich organelles universally found in most cells. They serve as a
key energy reservoir, actively participate in signal transduction and dynamically …
key energy reservoir, actively participate in signal transduction and dynamically …
Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes
In this work, we demonstrate the applicability of coherent anti-Stokes Raman scattering
(CARS) micro-spectroscopy for quantitative chemical imaging of saturated and unsaturated …
(CARS) micro-spectroscopy for quantitative chemical imaging of saturated and unsaturated …