Teleost fish possess an adaptive immune system associated with each of their mucosal
body surfaces. Evidence obtained from mucosal vaccination and mucosal infection studies …

Efforts to determine the antibody repertoire encoded by B cells in the blood or lymphoid
organs using high-throughput DNA sequencing technologies have been advancing at an …

Germinal centers (GCs) or analogous secondary lymphoid microstructures (SLMs) are
thought to have evolved in endothermic species. However, living representatives of their …

The intestinal mucosa contains the largest population of antibody-secreting plasma cells in
the body, and in humans several grams of secretory immunoglobulin A (SIgA) are released …

Immunoglobulins (Igs) are complex glycoproteins that play critical functions in innate and
adaptive immunity of all jawed vertebrates. Given the unique characteristics of mucosal …

Meiotic recombination and de novo mutation are the two main contributions toward gamete
genome diversity, and many questions remain about how an individual human's genome is …

The human antibody repertoire is one of the most important defenses against infectious
disease, and the development of vaccines has enabled the conferral of targeted protection …

High-throughput sequencing analysis of hypermutating immunoglobulin (IG) repertoires
remains a challenging task. Here we present a robust protocol for the full-length profiling of …

Stochastic rearrangement of germline V-, D-, and J-genes to create variable coding
sequence for certain cell surface receptors is at the origin of immune system diversity. This …

Antibody repertoire diversity and plasticity is crucial for broad protective immunity.
Repertoires change in size and diversity across multiple B cell developmental stages and in …