High-sensitivity analytical approaches for the structural characterization of glycoproteins
1.1. General Considerations Structural intricacies of carbohydrate molecules and their
propensity to form varied linkages, substitutions, and branching patterns have fascinated …
propensity to form varied linkages, substitutions, and branching patterns have fascinated …
Clinical and pharmaceutical applications of affinity ligands in capillary electrophoresis: A review
Affinity capillary electrophoresis (ACE) is a separation technique that combines a
biologically-related binding agent with the separating power and efficiency of capillary …
biologically-related binding agent with the separating power and efficiency of capillary …
Capillary electrophoresis–mass spectrometry for direct structural identification of serum N-glycans
Through direct coupling of capillary electrophoresis (CE) to mass spectrometry (MS) with a
sheathless interface, we have identified 77 potential N-glycan structures derived from …
sheathless interface, we have identified 77 potential N-glycan structures derived from …
Recent advances in capillary gel electrophoresis for the analysis of proteins
R Bhimwal, RR Rustandi, A Payne, M Dawod - Journal of Chromatography …, 2022 - Elsevier
The purpose of this review is to highlight noteworthy advancements in the field of capillary
gel electrophoresis for the separation and analysis of proteins from the period of 2015-2021 …
gel electrophoresis for the separation and analysis of proteins from the period of 2015-2021 …
[HTML][HTML] Automated N-glycosylation sequencing of biopharmaceuticals by capillary electrophoresis
M Szigeti, A Guttman - Scientific reports, 2017 - nature.com
Comprehensive analysis of the N-linked carbohydrates of glycoproteins is gaining high
recent interest in both the biopharmaceutical and biomedical fields. In addition to high …
recent interest in both the biopharmaceutical and biomedical fields. In addition to high …
Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis
G Lu, LA Holland - Analytical chemistry, 2018 - ACS Publications
Glycosylated human IgG contains fucosylated biantennary N-glycans with different
modifications including N-acetylglucosamine, which bisects the mannose core. Although …
modifications including N-acetylglucosamine, which bisects the mannose core. Although …
Unraveling the glyco-puzzle: glycan structure identification by capillary electrophoresis
S Mittermayr, J Bones, A Guttman - Analytical chemistry, 2013 - ACS Publications
State-of-the-art high-resolution separation techniques play an important role in the full
structural elucidation of glycans. Capillary electrophoresis (CE) offers a rapid yet simple …
structural elucidation of glycans. Capillary electrophoresis (CE) offers a rapid yet simple …
Separation of glycoproteins based on sugar chains using novel stationary phases modified with poly (ethylene glycol)-conjugated boronic-acid derivatives
H Kobayashi, Y Masuda, H Takaya, T Kubo… - Analytical …, 2022 - ACS Publications
Boronic acid (BA) reversibly complexes with the diol structure. BA derivatives separate
glycoproteins based on the differences in the sugar chains. Separation typically occurs …
glycoproteins based on the differences in the sugar chains. Separation typically occurs …
Structural characterization of serum N-glycans by methylamidation, fluorescent labeling, and analysis by microchip electrophoresis
I Mitra, CM Snyder, X Zhou, MI Campos… - Analytical …, 2016 - ACS Publications
To characterize the structures of N-glycans derived from human serum, we report a strategy
that combines microchip electrophoresis, standard addition, enzymatic digestion, and matrix …
that combines microchip electrophoresis, standard addition, enzymatic digestion, and matrix …
Comparative profiling of N-glycans isolated from serum samples of ovarian cancer patients and analyzed by microchip electrophoresis
I Mitra, WR Alley Jr, JA Goetz, JA Vasseur… - Journal of proteome …, 2013 - ACS Publications
Ovarian cancer is the fifth leading cause of cancer-related mortalities for women in the
United States and the most lethal gynecological cancer. Aberrant glycosylation has been …
United States and the most lethal gynecological cancer. Aberrant glycosylation has been …